EC Number |
Protein Variants |
Reference |
---|
1.1.98.6 | C260S |
activity comparable to wild-type, mutant is able to undergo truncation at the site of the glycyl radical when the radical-containing enzyme is exposed to oxygen |
740666 |
1.1.98.6 | C290S |
mutation in conserved residua, less than 10% of wild-type activity |
740662 |
1.1.98.6 | C290S |
residue participates in the reaction mechanism by forming a transient thiyl radical. Mutant is able to undergo truncation at the site of the glycyl radical when the radical-containing enzyme is exposed to oxygen |
740666 |
1.1.98.6 | C453S |
activity comparable to wild-type, mutant is able to undergo truncation at the site of the glycyl radical when the radical-containing enzyme is exposed to oxygen |
740666 |
1.1.98.6 | C543S |
residue is essential for formation of the glycyl radical |
740666 |
1.1.98.6 | C546S |
residue is essential for formation of the glycyl radical |
740666 |
1.1.98.6 | C561S |
residue is essential for formation of the glycyl radical |
740666 |
1.1.98.6 | C564S |
residue is essential for formation of the glycyl radical |
740666 |
1.1.98.6 | C579S |
mutant is able to undergo truncation at the site of the glycyl radical when the radical-containing enzyme is exposed to oxygen |
740666 |
1.1.98.6 | C644A |
mutation in a residue of the Zn(Cys)4 center, almost complete loss of activity due to inability to generate the catalytically essential glycyl radical |
660352 |