additional information |
the enzyme displays two nucleotide-binding sites. One site exhibits half-maximal saturation at approximately 5 mM 8-azidoadenosine 5'-triphosphate, whereas the other site requires 45 microM. The higher affinity site corresponds to residues 289-291 and the other site to the region to residues 147-160. Photoinsertion of 8-azidoadenosine 5'-triphosphate into the site corresponding to residues 147-160 is almost completely abolished when 100 mM dATP, dGTP, or dTTP is included in the photolabeling reaction mixture, whereas 100 mM ATP, GTP, CTP, or dCTP have virtually no effect |
Tequatrovirus T4 |
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