EC Number |
Protein Variants |
Reference |
---|
1.1.1.103 | C38D |
site-directed mutagenesis |
285702 |
1.1.1.103 | C38S |
1 cysteine residue per subunit is essential for catalytic activity and Mn2+ binding |
285704 |
1.1.1.103 | C38S |
site-directed mutagenesis, catalytically inactive mutant, C38 is located in an activating divalent metal-ion binding site |
285702 |
1.1.1.103 | D179A |
mutation affects switching between the open and closed form of the enzyme. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 36fold lower than wild-type value |
762728 |
1.1.1.103 | D179N |
mutation affects switching between the open and closed form of the enzyme. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 190fold lower than wild-type value |
762728 |
1.1.1.103 | D180A |
228fold decrease in the catalytic efficiency |
-, 762551 |
1.1.1.103 | E152A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
687461 |
1.1.1.103 | E152C |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
687461 |
1.1.1.103 | E152D |
site-directed mutagenesis, the E152D mutant shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD+ compared to wild-type TDH, Glu152 to Asp substitution causes the enhancement of deprotonation of His47 or ionization of zinc-bound water and threonine in the enzyme-NAD+ complex |
687461 |
1.1.1.103 | E152K |
site-directed mutagenesis, inactive mutant |
687461 |