EC Number |
Protein Variants |
Reference |
---|
4.2.1.30 | I498W |
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type |
704770 |
4.2.1.30 | more |
the linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit shows a 20°C increase in its temperature optimum |
747370 |
4.2.1.30 | Q42A |
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type |
704770 |
4.2.1.30 | Q42C |
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type |
704770 |
4.2.1.30 | Q42D |
most affected, alpha-helix around 152 positions is totally distorted. Higher optimal pH as the wild-type, lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type |
704770 |
4.2.1.30 | Q42E |
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type |
-, 704770 |
4.2.1.30 | Q42F |
most active one, catalytic activity in beta-subunit is 8.3fold higher than the wild-type. Higher optimal pH and higher optimal temperature as the wild-type. Higher KM for glycerol as the wild-type |
704770 |
4.2.1.30 | Q42G |
active, lower optimal pH and lower optimal temperature as the wild-type. KM for glycerol comparable to the wild-type |
704770 |
4.2.1.30 | Q42H |
active, optimal pH as the wild-type, higher optimal temperature as the wild-type. KM for glycerol comparable to the wild-type |
704770 |
4.2.1.30 | Q42I |
active, lower optimal pH and lower optimal temperature as the wild-type. Higher KM for glycerol as the wild-type |
704770 |