Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.30 extracted from

  • Maddock, D.J.; Gerth, M.L.; Patrick, W.M.
    An engineered glycerol dehydratase with improved activity for the conversion of meso-2,3-butanediol to butanone (2017), Biotechnol. J., 12, 1700480 .
    View publication on PubMed

Application

Application Comment Organism
synthesis synthesis of butanone Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
-
Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information the linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit shows a 20°C increase in its temperature optimum Klebsiella pneumoniae
T200S 4fold increase in catalytic efficiency of meso-2,3-butanediol dehydration Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
(R)-1,2-Propanediol 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae
0.7
-
meso-2,3-butanediol 37°C, pH 7.0, mutant enzyme T200S Klebsiella pneumoniae
2.6
-
meso-2,3-butanediol 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q59476 AND O08505 AND Q59475 Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1,2-propanediol
-
Klebsiella pneumoniae propionaldehyde + H2O
-
?
meso-2,3-butanediol
-
Klebsiella pneumoniae butanone + H2O
-
?

Subunits

Subunits Comment Organism
hexamer alpha,beta,gamma Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
KpGDHt
-
Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
optimum temperature (Topt) for the unlinked enzyme Klebsiella pneumoniae
57
-
linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 57 37°C: about 60% of maximal activity, 57°C: optimum, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
meso-2,3-butanediol 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae
3.6
-
meso-2,3-butanediol 37°C, pH 7.0, mutant enzyme T200S Klebsiella pneumoniae
200
-
(R)-1,2-Propanediol 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1
-
meso-2,3-butanediol 37°C, pH 7.0, mutant enzyme T200S Klebsiella pneumoniae
130
-
meso-2,3-butanediol 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae
390
-
(R)-1,2-Propanediol 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit Klebsiella pneumoniae