Application | Comment | Organism |
---|---|---|
synthesis | synthesis of butanone | Klebsiella pneumoniae |
Cloned (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit shows a 20°C increase in its temperature optimum | Klebsiella pneumoniae |
T200S | 4fold increase in catalytic efficiency of meso-2,3-butanediol dehydration | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
(R)-1,2-Propanediol | 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae | |
0.7 | - |
meso-2,3-butanediol | 37°C, pH 7.0, mutant enzyme T200S | Klebsiella pneumoniae | |
2.6 | - |
meso-2,3-butanediol | 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q59476 AND O08505 AND Q59475 | Q59476: alpha-subunit, O08505: beta-subunit, Q59475: gamma-subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
meso-2,3-butanediol | - |
Klebsiella pneumoniae | butanone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha,beta,gamma | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
KpGDHt | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
optimum temperature (Topt) for the unlinked enzyme | Klebsiella pneumoniae |
57 | - |
linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 57 | 37°C: about 60% of maximal activity, 57°C: optimum, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
meso-2,3-butanediol | 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae | |
3.6 | - |
meso-2,3-butanediol | 37°C, pH 7.0, mutant enzyme T200S | Klebsiella pneumoniae | |
200 | - |
(R)-1,2-Propanediol | 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
meso-2,3-butanediol | 37°C, pH 7.0, mutant enzyme T200S | Klebsiella pneumoniae | |
130 | - |
meso-2,3-butanediol | 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae | |
390 | - |
(R)-1,2-Propanediol | 37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit | Klebsiella pneumoniae |