EC Number |
Protein Variants |
Reference |
---|
3.7.1.3 | D201E |
mutation results in reduced catalytic activity with L-kynurenine and beta-benzoyl-L-alanine, but not O-benzoyl-L-serine. The mutant enzyme can form quinonoid and vinylogous amide intermediates with beta-benzoyl-L-alanine, similar to wild-type enzyme. The mutant enzyme reacts more slowly with beta-benzoyl-L-alanine and benzaldehyde to form an aldol product absorbing at 490 nm than wild-type |
756117 |
3.7.1.3 | H102W/N333T |
the mutant shows complete reversal of substrate specificity between 3-hydroxy-L-kynurenine and L-kynurenine |
699466 |
3.7.1.3 | H102W/S332G/N333T |
the mutant shows complete reversal of substrate specificity between 3-hydroxy-L-kynurenine and L-kynurenine |
699466 |
3.7.1.3 | more |
cosmid pRA3 corrects trpA mutants of Rhizobium leguminosarum |
673365 |
3.7.1.3 | more |
cosmid pRA3 corrects trpA, trpB and trpC mutants in Escherichia coli |
673365 |
3.7.1.3 | N333T |
the mutant reveals a 9fold decrease in kcat for L-kynurenine, but no change in Km, the mutant has weaker 3-hydroxy-L-kyrenine binding (6fold higher Km) and kcat at least 1100times slower than wild type Kynase |
699466 |
3.7.1.3 | S332G/N333T the |
mutant shows a very slight preference for L-kynurenine over 3-hydroxy-L-kynurenine |
699466 |
3.7.1.3 | S36A |
the mutant enzyme can form quinonoid and vinylogous amide intermediates with beta-benzoyl-L-alanine, similar to wild-type enzyme. Mutation results in a 230fold reduction of kcat and 30fold reduction in kcat/Km with L-kynurenine, but very little effect on the reaction of O-benzoyl-L-serine. The rate-determining step in the reaction of thje mutant enzyme the Cbeta-Cgamma bond cleavage |
756117 |
3.7.1.3 | T102I |
the mutation leads to a marked decrease in kynureninase activity and causes the red body coloration in the Bombyx mori red blood strain |
698102 |
3.7.1.3 | T198A |
screening of cDNA from KYNU revealed homozygosity for a c.593A>G substitution in exon 7 in the proband, leading to a threonine-to-alanine shift, T198A, in kynureninase |
688175 |