EC Number |
Protein Variants |
Reference |
---|
1.14.18.5 | H274A |
complete loss of activity |
733375 |
1.14.18.5 | L196W |
complete loss of activity |
733375 |
1.14.18.5 | more |
the replacement of any one of conserved His residues of three histidine-rich motifs with an alanine eliminates hydroxylase activity in vivo and in vitro. Residues Phe 174, Asn 182, Ser 191, Leu 196, Pro 199, Asn 266, Tyr 269, Asp 271 and Gln 275 appear to be additionally important elements of the active site but their conversion into corresponding yeast DELTA7-sterol-C5(6)-desaturase Erg3p residues does not lead to a gain in desaturase activity |
733375 |
1.14.18.5 | N182P |
complete loss of activity |
733375 |
1.14.18.5 | N266G |
complete loss of activity |
733375 |
1.14.18.5 | P199C |
complete loss of activity |
733375 |
1.14.18.5 | Q275L |
complete loss of activity |
733375 |
1.14.18.5 | S191K |
99% of wild-type activity |
733375 |
1.14.18.5 | Y269C |
complete loss of activity |
733375 |