EC Number   |
Protein Variants |
Reference |
|---|
    1.13.11.40 | R182A |
site-directed mutagenesis, the mutant shows reduced activity and altered protein fold compared to the wild-type enzyme |
742865 |
| 1.13.11.40 | R185A |
stability of the enzyme-substrate complex is similar to wild-type. Contrary to wild-type, hydrogen abstraction from C13 is more favorable in the mutant. R185A yields 87% 8R product, 2% 8S plus some 11R/11S, 12R/12S and 15R/15S product |
765268 |
| 1.13.11.40 | W41A |
140% activity compared to the wild type enzyme, exhibits only less than 2% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal |
674505 |
| 1.13.11.40 | W77A |
44% of the activity of the wild type enzyme, exhibits only 4% of the increase in fluorescence at 517 nm upon the addition of CaCl2 of the wild-type signal |
674505 |
| 1.13.11.40 | Y603F |
8-LOX mutant, is relative insensitive towards pH alterations in the near physiological range (pH 6-8), at strong alkaline conditions (pH more than 9) significant shares of 15S-H(p)ETE are formed |
702435 |
| 1.13.11.40 | Y603F/H604F |
8-LOX double mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product |
702435 |
