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Literature summary for 1.13.11.40 extracted from
- Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms (2009), Biochim. Biophys. Acta, 1791, 827-835.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H604F | 8-LOX mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product. Specific activity of 8-LOX mutant with (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid as substrate continously declines when pH increases | Mus musculus |
| Y603F | 8-LOX mutant, is relative insensitive towards pH alterations in the near physiological range (pH 6-8), at strong alkaline conditions (pH more than 9) significant shares of 15S-H(p)ETE are formed | Mus musculus |
| Y603F/H604F | 8-LOX double mutant, induces strong pH-dependent alterations in the positional specificity but the pH-optimum remains the same. At acidic pH 8S-H(p)ETE is the exclusive arachidonic acid oxygenation product but with more alkaline pH increasing shares of 15S-H(p)ETE, above pH 9 15S-H(p)ETE is the major oxygenation product | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on Ni column | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skin | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity of 8-LOX with (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid as substrate strongly increases going from pH 6 to 7.2 but then drops down at more alkaline pH | Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 10% glycerol | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid + O2 | 8-LOX tolerates a carboxylic group in the substrate-binding pocket | Mus musculus | ? | - |
? |  |
| arachidonate + O2 | - |
Mus musculus | (5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate | 8-H(p)ETE is the major reaction product of 8-LOX independent of the pH | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 8-lipoxygenase | - |
Mus musculus |
| 8-LOX | - |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
8-LOX | Mus musculus |
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Release 2023.1 (January 2023)
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