EC Number   |
|---|
   5.6.2.2 | crystal structure analysis, overview |
| 5.6.2.2 | determination of crystal structure of fragments |
| 5.6.2.2 | first report of crystallization and preliminary X-ray analysis of the DNA-cleavage domain of a topoisomerase IV from a gram positive organism. Sparse-matrix screening used, Crystals of both protein fragments (GrlA56 and GrlA59) display a plate-like morphology and initially grow in tightly packed clusters. Crystallization and X-ray analysis of two different constructs of the A subunit of topoisomerase IV from Staphylococcus aureus (GrlA) are described. With a view to gaining further insight into the mode of inhibition of quinolone antibiotics against this enzyme |
| 5.6.2.2 | hanging drop vapour diffusion method, in complex with the radicicol inhibitor |
| 5.6.2.2 | hanging-drop method at 19°C, crystal structure is determined at 4.0 A resolution. The 220000 Da heterotetramer adopts a twin-gate architecture, in which a pair of ATPase domains at one end of the enzyme is poised to coordinate DNA movements into the enzyme and through a set of DNA-cleaving domains at the other end |
| 5.6.2.2 | homology-based structure model of segment of amino acids 724-771 forming the alpha2 helix turn helix module with a compact alpha2betaalpha3talpha4 conformation. The helical content of human alpha2 helix turn helix module in solution is similar to that of its couterpart with yeast topoiaomerase II in the solid state. The segment self-associates into dimers. alpha2 helix turn helix module, helix turn helix and alpha4, but not alpha3 segments, bind effectively to DNA |
| 5.6.2.2 | molecular model of the entire enzyme in complex with DNA after the cleavage reaction. Anthracycline antibiotics contact specifically the cleaved DNA as well as amino acid residues of the enzyme CAP-like domain |
| 5.6.2.2 | recombinant engineered DNA gyrase GyrBA57 in apo- and AMPPNP-bound forms, sitting-drop vapour diffusion method, mixing of 10 mg/mlGyrBA57 in presence or absence of Mg2+ or 5 mM MgCl2 plus 2.5 mM AMPPNP, or in the presence of 1 mM AMPPNP plus 8 mM oligonucleotides, with 100 mM sodium acetate, 100 mM MES, pH 6.5, 26% PEG 400, 25% ethylene glycol, and 10 mM MgCl2 (for Apo-GyrBA57 and AMPPNP-bound GyrBA57, respectively), X-ray diffraction structure determination and analysis at 2.6 A and 3.3 A resolution, respectively |
| 5.6.2.2 | recombinant, improved for crystallization, DNA gyrase construct GyrB27-A56(GKdel/Tyr123Phe) in a ternary complex with a 20-bp double-nicked DNA and inhibitor GSK299423 or inhibitor ciprofloxacin, X-ray diffraction structure determination and analysis at 2.1 A and 3.35 A resolution, respectively |
| 5.6.2.2 | sitting drop method, crystal structure of the enzyme in the presence and absence of ATP |
