EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.4.16.5 | -999 |
- |
when the enzyme is treated at pressures higher than 300 mPa and temperatures lower than -5°C, it undergoes an irreversible inactivation in which nearly 50% of the alpha-helical structure is lost as judged by circular dichroism spectral analysis. When the applied pressure is limited to below 200 mPa, the cold inactivation process appears to be reversible. In the presence of reducing agent, this reversible phenomenon, observed at below 200 mPa, diminishes to give an inactive enzyme |
647230 |
3.4.16.5 | -30 |
20 |
30 min, 0.1 mPa, nearly full activity is maintained |
647230 |
3.4.16.5 | 10 |
- |
400 mPa, 10 min, 50% loss of activity |
647230 |
3.4.16.5 | 25 |
- |
pH 5.5-8, stable |
647189 |
3.4.16.5 | 37 |
- |
pH 6-8, stable for 2 h |
647195 |
3.4.16.5 | 45 |
50 |
the enzyme activity remained intact at temperatures below 45°C, but declined rapidly at temperatures exceeding 50°C |
679935 |
3.4.16.5 | 50 |
- |
pH 5.0, 15 min, stable up to |
665930 |
3.4.16.5 | 50 |
- |
pH 6.0, 15 min, isoenzyme MpiCP-2 is stable up to |
665932 |
3.4.16.5 | 55 |
- |
pH 6.0, 15 min, isoenzyme MpiCP-1 is stable |
665932 |
3.4.16.5 | 55 |
- |
unstable above |
665235 |