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Literature summary for 3.4.16.5 extracted from

  • Komai, T.; Kawabata, C.; Tojo, H.; Gocho, S.; Ichishima, E.
    Purification of serine carboxypeptidase from the hepatopancreas of Japanese common squid Todarodes pacificus and its application for elimination of bitterness from bitter peptides (2007), Fish. Sci., 73, 404-411.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1-chloro-3-tosylamido-2-heptanone 10% increase of activity at 10 mM Todarodes pacificus
antipain 15 increase of activity at 0.1 mM Todarodes pacificus
EDTA 25% increase of activity at 10 mM Todarodes pacificus
leupeptin 20% increase of activity at 0.1 mM Todarodes pacificus
N-ethylmaleimide 8% increase of activity at 10 mM Todarodes pacificus
o-phenanthroline 4% increase of activity at 5 mM Todarodes pacificus
pepstatin A 5% increase of activity at 0.1 mM Todarodes pacificus

Protein Variants

Protein Variants Comment Organism
nutrition the enzyme can be used to eliminate the bitterness of bitter peptides Todarodes pacificus

Inhibitors

Inhibitors Comment Organism Structure
(S)-1-chloro-4-phenyl-3-tosylamido-2-butanone 37% residual activity at 1 mM Todarodes pacificus
CaCl2 73% residual activity at 10 mM Todarodes pacificus
chymostatin 5% residual activity at 1 mM Todarodes pacificus
CoSO4 62% residual activity at 10 mM Todarodes pacificus
CuSO4 4% residual activity at 10 mM Todarodes pacificus
diisopropylfluorophosphate complete inhibition at 5 mM, at 30°C and pH 3.1 Todarodes pacificus
HgCl2 complete inhibition at 1 mM, at 30°C and pH 3.1 Todarodes pacificus
Monoiodoacetic acid complete inhibition at 1 mM, at 30°C and pH 3.1 Todarodes pacificus
N-(N-[1-trans-3-carboxyoxiran-2-carbonyl]-L-leucyl)-agmatine 93% residual activity at 1 mM Todarodes pacificus
p-chloromercuribenzoic acid complete inhibition at 1 mM, at 30°C and pH 3.1 Todarodes pacificus
Pb(CH3COO)2 60% residual activity at 10 mM Todarodes pacificus
Phenylmethylsulfonylfluoride 48% residual activity at 1 mM Todarodes pacificus
ZnSO4 69% residual activity at 10 mM Todarodes pacificus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
SDS-PAGE Todarodes pacificus
42000
-
gel filtration Todarodes pacificus

Organism

Organism UniProt Comment Textmining
Todarodes pacificus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono S cation exchange column chromatography and Superdex 75 gel filtration Todarodes pacificus

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
Todarodes pacificus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Glu-Phe + H2O 150% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Glu + Phe
-
?
Benzyloxycarbonyl-Glu-Tyr + H2O 100% activity Todarodes pacificus Benzyloxycarbonyl-Glu + Tyr
-
?
Benzyloxycarbonyl-Gly-Leu + H2O 17% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus Benzyloxycarbonyl-Gly + Leu
-
?
benzyloxycarbonyl-Gly-Met + H2O 19% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Gly + Met
-
?
Benzyloxycarbonyl-Gly-Phe + H2O 21% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus Benzyloxycarbonyl-Gly + Phe
-
?
benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O 309% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Gly-Pro-Leu + Gly
-
?
benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O 3% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Gly-Pro-Leu-Gly + Pro
-
?
Benzyloxycarbonyl-Gly-Val + H2O 24% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus Benzyloxycarbonyl-Gly + Val
-
?
benzyloxycarbonyl-Phe-Ala + H2O 219% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Phe + Ala
-
?
benzyloxycarbonyl-Phe-Leu + H2O 483% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Phe + Leu
-
?
benzyloxycarbonyl-Phe-Pro + H2O 12% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Phe + Pro
-
?
Benzyloxycarbonyl-Phe-Tyr + H2O 114% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus Benzyloxycarbonyl-Phe + Tyr
-
?
benzyloxycarbonyl-Phe-Tyr-Gly + H2O 172% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Phe-Tyr + Gly
-
?
benzyloxycarbonyl-Phe-Tyr-Leu + H2O 120% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Phe-Tyr + Leu
-
?
benzyloxycarbonyl-Tyr-Gly + H2O 188% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Tyr + Gly
-
?
benzyloxycarbonyl-Tyr-Phe + H2O 201% activity compared to benzyloxycarbonyl-Glu-Tyr Todarodes pacificus benzyloxycarbonyl-Tyr + Phe
-
?
corn gluten + H2O
-
Todarodes pacificus ?
-
?
additional information no activity towards benzyloxycarbonyl-Gly-Pro, benzoyl-Gly-Lys, and benzyloxycarbonyl-Pro-Pro Todarodes pacificus ?
-
?
pepsin-digested soy protein + H2O
-
Todarodes pacificus ?
-
?
trypsin-digested casein + H2O
-
Todarodes pacificus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Todarodes pacificus
monomer 1 * 42000, gel filtration Todarodes pacificus

Synonyms

Synonyms Comment Organism
acidic serine carboxypeptidase
-
Todarodes pacificus
CPase
-
Todarodes pacificus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Todarodes pacificus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 50 the enzyme activity remained intact at temperatures below 45°C, but declined rapidly at temperatures exceeding 50°C Todarodes pacificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Todarodes pacificus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 6 at pH 3.1, its relative activity is maintained (70%), the enzyme remains stable in the pH range of 2-6 at 30°C for 30 min Todarodes pacificus

pI Value

Organism Comment pI Value Maximum pI Value
Todarodes pacificus isoelectric focusing
-
6