EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.2.1.22 | 40 |
55 |
purified recombinant wild-type enzyme is stable at 40°C after incubation for 1 h, but its stability decreases rapidly above 40°C. The mutants N286E and N286H show the same stability, decreasing rapidly above 40°C and exhibiting 20% and 40% of their maximum activity at 45°C, respectively. In contrast, mutant N286T is stable above 50°C and retains 60% of its maximum activity at 55°C after incubation for 1 h |
742119 |
1.2.1.22 | 50 |
- |
pH 6.5, 10 min, 50% loss of activity |
288132 |
1.2.1.22 | 55 |
- |
1 h, 70% residual activity for mutant N286T |
740187 |
1.2.1.22 | 80 |
- |
86% activity after 80°C for 10 min |
674270 |
1.2.1.22 | 90 |
- |
45% activity after 90°C for 10 min |
674270 |
1.2.1.22 | 100 |
- |
25% activity after 100°C for 10 min |
674270 |