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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 4.2.3.86construction of the synthetic terpene synthase construct from the sequence of alpga-guaiene synthase (EC 4.2.3.87), sequence comparisons and phylogenetic analysis, the recombinant enzyme is transiently expressed in Nicotiana benthamiana leaves and produces mainly alpha-guaiene and alpha-bulnesene, and also lower amounts of alpha-copaene, alpha-gurjunene, gamm-gurlunene, epiglobulol, epi-?alpha-selinene, and pogostol. Selina-4(14),11-diene, alpha-selinene, delta-cadinene, and intermedeol are not found
Display the reaction diagram Show all sequences 4.2.3.86expression in Escherichia coli
Display the reaction diagram Show all sequences 4.2.3.86His-tagged VvValCS is expressed in Escherichia coli
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