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EC Number	Cloned (Commentary)
2.4.1.148	functional expression in CHO cells, soluble chimeric enzyme form shows core 4 6beta-GalNAc transferase activity, DNA and amino acid sequence determination
2.4.1.148	mutant enzyme C217S is expressed in HEK-293T cells
2.4.1.148	phylogenetic analysis, the gene Bo17, encoding the enzyme, is acquired from an ancestor of the African buffalo 1.5 million years ago
2.4.1.148	stable expression of the enzyme, lacking the cytoplasmic tail and the transmembrane domain, in CHO cells, secretion of the fully active recombinant enzyme to the culture medium

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Release 2023.1 (January 2023)