EC Number   |
|---|
   1.14.14.81 | DNA and amino acid sequence determination and analysis, genetic mapping, phylogenetic analysis, expression analysis and metabolic profiling, phenotypes |
| 1.14.14.81 | DNA and amino acid sequence determination and analysis, phylogenetic analysis of sequences of both enzymes indicate that F3',5'H is recruited from F3'H before the divergence of angiosperms and gymnosperms, overview |
| 1.14.14.81 | DNA and amino acid sequence determination and analysis, sequence comparison, genetic organization, and phylogenetic analysis |
| 1.14.14.81 | DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme overexpression in Petunia hybrida hybrid M1 x R27, which contains a mutated endogenous F3'5'H (HF1), no obvious phenotype |
| 1.14.14.81 | expression in Dendrathemum grandiflora transgenic plants using the Agrobacterium tumefaciens stran ABA 4404 transfection method |
| 1.14.14.81 | expression in Escherichia coli JM109 |
| 1.14.14.81 | expression in Petunia hybrida |
| 1.14.14.81 | expression in Rosa hybrida transgenic plants, the mutant plants show the accumulation of a high percentage of delphinidin in selected cultuvars, while the wild-type lacks delphinidin and derivatives and the activity of F3'5'H |
| 1.14.14.81 | expression of wild-type enzyme and chimeric mutants in yeast strain INVSc 1 microsomes |
| 1.14.14.81 | functional expression of the enzyme as fusion enzyme with a P450 reductase leading to biosynthesis of plant-specific di- and trihydroxylated flavonols in Escherichia coli strain BL21(DE3), feeding experiments and determination of the flavonoid spectra in different recombinant bacterial lines in vivo, overview |
