EC Number   |
|---|
   1.14.11.21 | gene cs2, isozyme CS2, wild-type and mutants, overexpression in Escherichia coli JM101 |
| 1.14.11.21 | isozymes CAS1 and CAS2 encoded by genes cas1 and cas2, respectively, genetic analysis |
| 1.14.11.21 | isozymes CS1 and CS2, overexpression in Escherichia coli JM101 |
| 1.14.11.21 | Streptomyces venezuelae YJ028 is utilized as the heterologous host for the expression of four structural clavulanic acid biosynthesis genes, which encode carboxyethylarginine synthase (ceas2), beta-lactam synthetase (bls2), clavaminate synthase (cas2), and proclavaminate amidinohydrolase (pah2). The genes are cloned into pIBR25 expression vector containing ermE promoter to generate pBS4. The cas2 gene is also cloned into pSET152 to generate pCas2. It is then integrated into the genome of Streptomyces venezuelae YJ028 |
