EC Number   |
|---|
   1.1.1.202 | gene dhaT, high level co-expression of the 1,3-PD oxidoreductase with glycerol dehydratase, encoded by gene dhaB, and glycerol dehydratase reactivating factor, encoded by gene gdrAB, in Escherichia coli strain BL21 (DE3) using two incompatible plasmids, fed-batch fermentation of recombinant bacteria. The NADPH-linked alcohol dehydrogenase, which is encoded by yqhD, a gene from Escherichia coli, can non-specifically catalyze 3-hydroxypropionaldehyde converting to 1,3-propanediol with sufficient activity, overview |
| 1.1.1.202 | gene dhaT, part of the dha regulon, genetic structure, overview. Coexpressions of the PDOR and GDHt from gene dhaB in Klebsiella pneumoniae result in an increase of molar yield from 50.6-64.0% of 1,3-propanediol |
| 1.1.1.202 | gene dhaT, part of the dha regulon, genetic structure, recombinant expression in Escherichia coli |
| 1.1.1.202 | gene dhaT, part of the dha regulon, recombinant expression in Escherichia coli |
| 1.1.1.202 | gene dhaT, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.1.1.202 | gene TM0920, expression of soluble N-terminally MGSDKIHHHHHH-tagged selenomethionine-labeled enzyme in Escherichia coli methionine auxotrophic strain DL41 |
| 1.1.1.202 | genes for the production of propane-1,3-diol in Clostridium butyricum, dhaB1 and dhaB2, which encode the vitamin B12-independent glycerol dehydratase DhaB1 and its activating factor, DhaB2, respectively, tandemly arrayed with the Escherichia coli yqhD gene, which encodes the 1,3-propanediol oxidoreductase isoenzyme YqhD. Heterologous expression of the propane-1,3-diol operon under the control of the temperature-sensitive lambda phage PLPR promoter regulated by the cIts857 repressor, from plasmid pDY220, in Escherichia coli K-12 ER2925 |
| 1.1.1.202 | overexpression in Escherichia coli |
| 1.1.1.202 | overexpression of gene dhaT encoding PDOR (from vector pETPkan-dhaT) and transformed into Klebsiella pneumoniae |
| 1.1.1.202 | plasmid including Escherichia coli yqhD introduced into Klebsiella pneumoniae AK mutant strain defective in in 1,3-PD oxidoreductase activity (DhaT) |
