EC Number   |
Substrates |
Organism |
Products |
Reversibility  |
|---|
   4.2.1.119 | (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O |
reaction of the recombinant enzyme, protein converted rapidly |
Rattus norvegicus |
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA |
a physiological intermediate in bile acid synthesis |
? |
| 4.2.1.119 | (2E)-2-decenoyl-CoA + H2O |
activity measurements are based on the formation of the magnesium complex of 3-ketoacyl-CoA from (2E)-2-decenoyl-CoA |
Candida tropicalis |
(3R)-3-hydroxydecanoyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-2-enoyl-CoA + H2O |
- |
Saccharomyces cerevisiae |
(3R)-3-hydroxyacyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-2-enoyl-CoA + H2O |
2-enoyl-CoA hydratase 2 is a part of multifunctional enzyme type 2, hydrates trans-2-enoyl-CoA to 3-hydroxyacyl-CoA as a key enzyme in the (3R)-hydroxy-dependent route of peroxisomal beta-oxidation of fatty acids |
Candida tropicalis |
(3R)-3-hydroxyacyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-2-enoyl-CoA + H2O |
peroxisomal multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units. It catalyzes the second and third steps of peroxisomal beta-oxidation, and its importance in human lipid metabolism is shown by the severe clinical symptoms (dysmorphic features, such as macrocephaly and large fontanelles, hypotonia, seizures, etc.) in patients having defects in the gene encoding MFE-2. Typical biochemical observations include a high ratio of C26:0 to C22:0 fatty acids and elevated levels of pristanic acid (2,6,10,14-tetramethylpentadecanoic acid) in the patients plasma and fibroblasts, indicating the significance of MFE-2 in the breakdown of very-long-chain and alpha-methylbranched-chain fatty acids. The patients also have high levels of di- and trihydroxycholestanoic acids, which are precursors of bile acids, showing that MFE-2 also participates in bile acid synthesis |
Homo sapiens |
(3R)-3-hydroxyacyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-butenoyl-CoA + H2O |
- |
Drosophila melanogaster |
(3R)-hydroxybutanoyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-decenoyl-CoA + H2O |
- |
Drosophila melanogaster |
(3R)-3-hydroxydecanoyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-decenoyl-CoA + H2O |
- |
Rattus norvegicus |
(3R)-3-hydroxydecanoyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-enoyl-CoA + H2O |
- |
Drosophila melanogaster |
(3R)-hydroxyacyl-CoA |
- |
? |
| 4.2.1.119 | (2E)-enoyl-CoA + H2O |
straight-chain |
Rattus norvegicus |
(3R)-hydroxyacyl-CoA |
- |
? |
