EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.2.2.22 | 14-mer rRNA with GAGA tetraloop + H2O |
substrate and product with specific DNA loop probe, fluorophore and quencher, 37°C, citrate buffer, pH 4.0, than neutralization to pH 7.6 and hybridization with DNA-probes |
Ricinus communis |
14-mer rRNA with GabGA + ? |
- |
? |
| 3.2.2.22 | 2'-anthraniloyl 7-methylguanosine triphosphate + H2O |
depurination downstream of cap structure, 2.4-fold enhanced binding to m7GTP upon the addition of translation initiation factor G4 (elFiso4G) |
Phytolacca americana |
? |
- |
? |
| 3.2.2.22 | 23S rRNA + H2O |
- |
Trichosanthes kirilowii |
? |
- |
? |
| 3.2.2.22 | 23S rRNA + H2O |
substrate is from Escherichia coli ribosomes, the naked 23S rRNA is a good substrate, while the intact native ribosome ins no substrate |
Ricinus communis |
? |
- |
? |
| 3.2.2.22 | 25S rNA + H2O |
- |
Phytolacca americana |
? |
enzyme depurinates the sarcin/ricin loop when the A-site of the ribosomal peptidyl-transferase center is unoccupied |
? |
| 3.2.2.22 | 25S rRNA + H2O |
depurination |
Escherichia coli |
? |
- |
? |
| 3.2.2.22 | 26S rRNA + H2O |
depurination of ribosomes from yeast, product determination using aniline coupling |
Ricinus communis |
? |
- |
? |
| 3.2.2.22 | 28S RNA + H2O |
substrate from rat liver ribosomes, depurination of A4324, generation of a 420nt fragment, product determination using aniline coupling |
Abrus precatorius |
? |
- |
? |
| 3.2.2.22 | 28S rRNA + H2O |
- |
Escherichia coli |
? |
- |
? |
| 3.2.2.22 | 28S rRNA + H2O |
- |
Zea mays |
? |
- |
? |
