EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.1.21.6 | covalently closed circular DNA pUC18 + H2O |
- |
Mycolicibacterium smegmatis |
? |
- |
? |
3.1.21.6 | Lambda DNA |
- |
Streptomyces glaucescens |
Double-stranded fragments between 7 and 24 bases with 5' and/or 3'-protruding single-stranded tails |
- |
? |
3.1.21.6 | lambda DNA + H2O |
- |
Mycolicibacterium smegmatis |
? |
- |
? |
3.1.21.6 | pBR322 DNA + H2O |
- |
Mycolicibacterium smegmatis |
DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively |
- |
? |
3.1.21.6 | more |
catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically |
Mycolicibacterium smegmatis |
? |
- |
? |
3.1.21.6 | double-stranded DNA |
preferably binds to the dinucleotide 5'-CC-3' and nicks DNA between both cytosines |
Streptomyces glaucescens |
? |
- |
? |
3.1.21.6 | 28S rDNA + H2O |
R1Bm EN cleaves both strands of the 28S rDNA sequence at the target site, 5'-A(G/C)(A/T)!(A/G)T-3' is the consensus sequence, thus cleavage site |
Bombyx mori |
? |
- |
? |
3.1.21.6 | more |
the enzyme does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA |
Virus NYs-1 |
? |
- |
? |
3.1.21.6 | double-stranded DNA |
the enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CIIIC-3' sequences but not 5'-IIICC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5-CC-3' sequences on opposite strands are close enough for the two strands to separate. When the 5'-CC-3' sequence on opposite strands are further apart only a portion of the strands separate |
Virus NYs-1 |
? |
- |
? |