the enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-CIIIC-3' sequences but not 5'-IIICC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5-CC-3' sequences on opposite strands are close enough for the two strands to separate. When the 5'-CC-3' sequence on opposite strands are further apart only a portion of the strands separate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
by hanging drop vapor diffusion method, to 2.0 A resolution, belongs to space group P321 with unit cell parameters a = b = 141.3 A, c = 37.5 A, alpha = beta = 90°, gamma = 120°, has a beta-hairpin region (residues 176-183) at the edge of the DNA binding surface