EC Number   |
Substrates |
Organism |
Products  |
Reversibility |
|---|
    1.5.3.1 | L-proline + O2 + H2O |
less than 1% the rate of sarcosine |
Bacillus sp. (in: Bacteria) |
? |
- |
r |
| 1.5.3.1 | more |
no substrate: choline,betaine, dimethylglycine and N-methyl amino acids |
Cylindrocarpon didymum |
? |
- |
? |
| 1.5.3.1 | more |
flavin and cytochromes of the c and b or o type function as electron carriers |
Bacteria |
? |
- |
? |
| 1.5.3.1 | more |
betaine |
Achromobacter denitrificans |
? |
- |
? |
| 1.5.3.1 | more |
not: beta-alanine, N-methylalanine, 1,3-dimethylurea, 1-methylguanidine, methoxyacetate, creatine, creatinine |
Bacillus sp. (in: Bacteria) |
? |
- |
? |
| 1.5.3.1 | more |
very specific for oxygen as acceptor, oxygen can be replaced by 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, much smaller Vmax/Km values than for O2 |
Corynebacterium sp. |
? |
- |
? |
| 1.5.3.1 | more |
dimethylglycine |
Achromobacter denitrificans |
? |
- |
? |
| 1.5.3.1 | more |
also oxidizes other amino acids containing a secondary amino group e.g. L-proline, and N-methyl-L-alanine |
Bacillus sp. (in: Bacteria) |
? |
- |
? |
| 1.5.3.1 | more |
heterotetrameric sarcosine oxidase is a flavoprotein that catalyses the oxidative demethylation of sarcosine to generate glycine, hydrogen peroxide and formaldehyde or 5,10-methylenetetrahydrofolate, depending on the availability of tetrahydrofolate. The amine proton of sarcosine is transferred to the unprotonated Lys residue in the enzyme-substrate complex |
Corynebacterium sp. |
? |
- |
? |
| 1.5.3.1 | more |
the alpha subunit shows sarcosine oxidase and L-proline dehydrogenase activity, while the beta subunit displays both sarcosine oxidase and L-proline dehydrogenase activity, but not NADH dehydrogenase activity, measurement of L-proline dehydrogenase activity with 2,6-dichloroindophenol as cofactor. Dye-linked NADH dehydrogenase activity is assayed with NADH, FAD and 2,6-dichloroindophenol |
Thermococcus kodakarensis |
? |
- |
? |
