EC Number   |
Substrates  |
Organism |
Products |
Reversibility |
|---|
   2.4.1.9 | more |
the truncated enzyme mutant IslA4 can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity, and a product specificity similar to the multidomain wild-type enzyme. High sucrose concentrations shift the specificity of the reaction towards fructooligosaccharides (FOS) synthesis, which almost eliminates inulin synthesis and leads to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allow for high levels of FOS synthesis, where 70% sucrose is used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS, quantitative product analysis, overview |
Leuconostoc citreum |
? |
- |
? |
| 2.4.1.9 | more |
the enzyme also hydrolyzes sucrose to D-fructose and D-glucose |
Limosilactobacillus reuteri |
? |
- |
- |
| 2.4.1.9 | more |
the enzyme also hydrolyzes sucrose to D-fructose and D-glucose |
Limosilactobacillus reuteri 121 |
? |
- |
- |
| 2.4.1.9 | more |
wild-type inulosucrase synthesizes mostly fructooligosaccharides up to a degree of polymerization of 15 and relatively low amounts of inulin polymer |
Limosilactobacillus reuteri 121 |
? |
- |
? |
| 2.4.1.9 | more |
the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose |
Limosilactobacillus reuteri 121 |
? |
- |
? |
| 2.4.1.9 | more |
sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside |
Lactobacillus gasseri DSM 20604 |
? |
- |
? |
| 2.4.1.9 | more |
enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview |
Lactobacillus gasseri DSM 20604 |
? |
- |
? |
| 2.4.1.9 | raffinose + [(2->1)-beta-D-fructosyl]n |
- |
Lactobacillus gasseri |
glucose + [(2->1)-beta-D-fructosyl]n+1 |
- |
? |
| 2.4.1.9 | raffinose + [(2->1)-beta-D-fructosyl]n |
- |
Lactobacillus gasseri DSM 20604 |
glucose + [(2->1)-beta-D-fructosyl]n+1 |
- |
? |
| 2.4.1.9 | sucrose + (2,1-beta-D-fructosyl)n |
- |
Limosilactobacillus reuteri |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 |
- |
? |
