Any feedback?
Information on EC 2.4.1.9 - inulosucrase
for references in articles please use BRENDA:EC2.4.1.9Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.4.1.9 inulosucraseIUBMB Comments
Converts sucrose into inulin and D-glucose. Some other sugars can act as D-fructosyl acceptors.
Specify your search results
Word Map
- 2.4.1.9
-
farnesyltransferase
-
farnesylation
-
prenylation
-
geranylgeranylation
-
isoprenoids
- fructooligosaccharides
- ggtase-i
- prenyltransferases
-
peptidomimetic
- h-ras
- inulin
-
transfructosylation
- levan
-
ca1a2x
-
15-carbon
- tipifarnib
- levansucrase
- rhob
-
p21ras
-
geranylgeranyltransferase-i
- reuteri
-
ggtis
-
inulin-type
- fructosyltransferases
- 1-kestose
- cvim
- fructansucrases
- biotechnology
- citreum
- lonafarnib
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
ftase, inulosucrase, isase, sucrose 1-fructosyltransferase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
fructansucrase
fructosyltransferase
fructosyltransferase, sucrose 1-
-
-
-
-
FTase
FTF
GH68 fructansucrase
HugO
INU
InuJ
IS
ISase
IslA
sucrose 1-fructosyltransferase
-
-
-
-
sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase
sucrose:2,1-beta-D-fructan1-beta-D-fructosyltransferase
additional information
sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase
-
-
sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase
-
-
sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase
-
additional information
-
enzyme belongs to the family 68 of glucoside hydrolases
additional information
enzyme belongs to the family 68 of glucoside hydrolases
additional information
-
enzyme belongs to the family 68 of glucoside hydrolases
-
additional information
enzyme belongs to the family 68 of glucoside hydrolases
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sucrose + [(beta-D-Fruf-(2->1))n]-alpha-D-Glup = alpha-D-glucose + [(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup
sucrose + [(beta-D-Fruf-(2->1))n]-alpha-D-Glup = alpha-D-glucose + [(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup
-
-
-
-
sucrose + [(beta-D-Fruf-(2->1))n]-alpha-D-Glup = alpha-D-glucose + [(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup
catalytic triad formed by essential residues D272, D424, and E523
-
sucrose + [(beta-D-Fruf-(2->1))n]-alpha-D-Glup = alpha-D-glucose + [(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup
catalytic triad formed by essential residues D272, D424, and E523
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
fructosyl group transfer
-
-
-
-
hexosyl group transfer
-
-
-
-
hydrolysis
transglycosylation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
sucrose:(2->1)-beta-D-fructan 1-beta-D-fructosyltransferase
Converts sucrose into inulin and D-glucose. Some other sugars can act as D-fructosyl acceptors.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9030-16-4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + sucrose
alpha-D-glucose + medium chain fructooligosaccharides (DP 2-20)
with 300 g/l sucrose, 128.4 g/l of fructooligosaccharide is produced with 85.6% conversion yield. From 400 g/l sucrose, 152.6 g/l of fructooligosaccharides is produced with 76.3% conversion yield
-
-
?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup
alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup
3 sucrose + sucrose
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
3 sucrose + sucrose
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(21) linkages]
-
-
?
3 sucrose + sucrose
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(21) linkages]
-
-
?
raffinose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
raffinose + [(2->1)-beta-D-fructosyl]n
glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin, determination of inulin product chain length, overview
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin, determination of inulin product chain length, overview
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
transferase activity
i.e. inulin
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
FTase possesses only transfructosylating activity, acts on sucrose by cleaving the beta-1,2 linkage and transferring the fructosyl group to an acceptor molecule such as sucrose and fructooligosaccharides, releasing D-glucose
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,1-beta-D-fructosyl)n
D-glucose + (2,1-beta-D-fructosyl)n+1
-
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity. Transglycosylation activity is higher in the whole concentration range tested and completely dominating at higher sucrose concentrations
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
hydrolytic activity
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
hydrolytic activity
-
-
?
sucrose + H2O
D-fructose + D-glucose
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose
-
-
?
sucrose + H2O
D-fructose + D-glucose
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose
-
-
?
sucrose + raffinose
?
synthesizes fructosylraffinose (most likely GalGF2) and a range of larger oligomers (up to GalGF6), and some polymeric material
-
-
?
sucrose + raffinose
?
synthesizes fructosylraffinose (most likely GalGF2) and a range of larger oligomers (up to GalGF6), and some polymeric material
-
-
?
sucrose + raffinose
glucose + 1-kestose
-
-
i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
-
?
sucrose + raffinose
glucose + 1-kestose
-
-
i.e. beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
-
?
sucrose + sucrose
1-kestose + nystose + fructooligosaccharides
-
-
-
-
?
sucrose + sucrose
1-kestose + nystose + fructooligosaccharides
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin nanoparticles
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin nanoparticles
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
Limosilactobacillus reuteri TMW1.106
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan polymer
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructan polymer
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructooligosaccharides
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type fructooligosaccharides
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
-
-
-
-
?
sucrose + sucrose
alpha-D-glucose + inulin-type oligosaccharide + inulin
Limosilactobacillus reuteri TMW1.106
-
-
-
?
sucrose + sucrose
kestose + nystose + fructooligosaccharides
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose. From the very early stage of the reaction, after 5 min incubation, traces of nystose are visible. The nystose concentration remains constant once it reached a similar level to kestose (after 1 h), and synthesis of FOS of a larger size starts. The enzyme synthesizes mainly a broad range of fructooligosaccharides of the inulin type in a non-processive reaction
-
-
?
sucrose + sucrose
kestose + nystose + fructooligosaccharides
at sucrose concentrations lower than 200 mM (at 37°C), hydrolysis is the main enzyme activity. At higher sucrose concentrations, transglycosylation increases gradually, reaching 90% or more of total enzyme activity at 1.7 M sucrose. From the very early stage of the reaction, after 5 min incubation, traces of nystose are visible. The nystose concentration remains constant once it reached a similar level to kestose (after 1 h), and synthesis of FOS of a larger size starts. The enzyme synthesizes mainly a broad range of fructooligosaccharides of the inulin type in a non-processive reaction
-
-
?
sucrose + sucrose
[(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose
-
transgylcosylation activity. Transglycosylation activity is higher in the whole concentration range tested and completely dominating at higher sucrose concentrations
-
-
?
sucrose + sucrose
[(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose
transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + sucrose
[(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose
transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C
-
-
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF5 from sucose
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF4 from sucose
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF4 from sucose
?
sucrose + sucrose
[(beta-D-Fruf-(2-1))5]-alpha-D-Glup + alpha-D-glucose
-
-
the enzyme selectively produces GF5 from sucose
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Aspergillus sydowii IAM 2544
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
Bacillus sp. 217C-11
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
?
sucrose + [(2->1)-beta-D-fructosyl]n
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
-
-
-
?