EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.4.22.8 | -999 |
- |
- |
81585, 81595 |
3.4.22.8 | 0.022 |
- |
commercial enzyme preparation, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C |
718358 |
3.4.22.8 | 0.039 |
- |
purified recombinant enzyme, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C |
718358 |
3.4.22.8 | 0.119 |
- |
culture supernatant, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C |
718358 |
3.4.22.8 | 0.172 |
- |
substrate benzoyl-L-Lys-4-nitroanilide |
709851 |
3.4.22.8 | 4.28 |
- |
substrate benzoyl-L-Arg-4-nitroanilide |
709851 |
3.4.22.8 | 25.45 |
- |
substrate Ac-L-Lys-4-nitroanilide |
709851 |
3.4.22.8 | 61.2 |
- |
recombinant enzyme after 515fold purification, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C |
718358 |
3.4.22.8 | 66 |
- |
- |
81591 |
3.4.22.8 | 123.5 |
- |
substrate benzoyl-L-Arg-4-nitroanilide |
709851 |