Cloned (Comment) | Organism |
---|---|
expressed in Clostridium perfringens strains 13 and 13DELTA6 | Hathewaya histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica | |
0.34 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14929 | - |
1 * 38061 + 1 * 14929, purified recombinant enzyme, MALDI-TOF mass spectrometry | Hathewaya histolytica |
14947 | - |
1 * 38067 + 1 * 14947, commercial enzyme preparation, MALDI-TOF mass spectrometry | Hathewaya histolytica |
14957 | - |
1 * 38091 + 1 * 14957, purified recombinant enzyme, calculated from amino acid sequence | Hathewaya histolytica |
15000 | - |
1 * 38000 + 1 * 15000, SDS-PAGE | Hathewaya histolytica |
38000 | - |
1 * 38000 + 1 * 15000, SDS-PAGE | Hathewaya histolytica |
38061 | - |
1 * 38061 + 1 * 14929, purified recombinant enzyme, MALDI-TOF mass spectrometry | Hathewaya histolytica |
38067 | - |
1 * 38067 + 1 * 14947, commercial enzyme preparation, MALDI-TOF mass spectrometry | Hathewaya histolytica |
38091 | - |
1 * 38091 + 1 * 14957, purified recombinant enzyme, calculated from amino acid sequence | Hathewaya histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya histolytica | P09870 | - |
- |
Hathewaya histolytica ATCC 19401 | P09870 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydroxyapatite column chromatography, and benzamidine-Sepharose column chromatography | Hathewaya histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.022 | - |
commercial enzyme preparation, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
0.039 | - |
purified recombinant enzyme, using acyl-L-lysine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
0.119 | - |
culture supernatant, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
61.2 | - |
recombinant enzyme after 515fold purification, using Nalpha-benzoyl-L-arginine-4-nitroanilide as substrate, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-L-lysine-4-nitroanilide + H2O | - |
Hathewaya histolytica | acyl-L-lysine + 4-nitroaniline | - |
? | |
acyl-L-lysine-4-nitroanilide + H2O | - |
Hathewaya histolytica ATCC 19401 | acyl-L-lysine + 4-nitroaniline | - |
? | |
Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O | the enzyme exhibits strict specificity for arginine at the P1 position, the enzyme displays 2fold higher specific activity with N-benzoyl-L-arginine-4-nitroanilide compared to acyl-L-lysine-4-nitroanilide | Hathewaya histolytica | Nalpha-benzoyl-L-arginine + 4-nitroaniline | - |
? | |
Nalpha-benzoyl-L-arginine-4-nitroanilide + H2O | the enzyme exhibits strict specificity for arginine at the P1 position, the enzyme displays 2fold higher specific activity with N-benzoyl-L-arginine-4-nitroanilide compared to acyl-L-lysine-4-nitroanilide | Hathewaya histolytica ATCC 19401 | Nalpha-benzoyl-L-arginine + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 38000 + 1 * 15000, SDS-PAGE | Hathewaya histolytica |
heterodimer | 1 * 38061 + 1 * 14929, purified recombinant enzyme, MALDI-TOF mass spectrometry | Hathewaya histolytica |
heterodimer | 1 * 38067 + 1 * 14947, commercial enzyme preparation, MALDI-TOF mass spectrometry | Hathewaya histolytica |
heterodimer | 1 * 38091 + 1 * 14957, purified recombinant enzyme, calculated from amino acid sequence | Hathewaya histolytica |
Synonyms | Comment | Organism |
---|---|---|
CLO | - |
Hathewaya histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.8 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica | |
69.5 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
116 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | commercial enzyme preparation, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica | |
234 | - |
Nalpha-benzoyl-L-arginine-4-nitroanilide | purified recombinant enzyme, in 50 mM Tris-HCl, 5 mM dithiothreitol, and 5 mM CaCl2, at pH 7.5 and 25°C | Hathewaya histolytica |