EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   2.5.1.2 | -999 |
- |
a method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. The assay is optimized for high-throughput analysis in a 96-well format with complex biological samples |
684401 |
| 2.5.1.2 | -999 |
- |
average thaminase activity is 42 microM per g and min. Gravid mussels show signifcantly lower thiaminase activity (28 microM /g and min) than non-gravid muscles (42 microM /g and min). Native freshwater mussel thiaminase activity is within the range observed for invasive dreissenids |
738782 |
| 2.5.1.2 | -999 |
- |
average thaminase activity is 7.2 microM per g and min. Native freshwater mussel thiaminase activity is within the range observed for invasive dreissenids |
738782 |
| 2.5.1.2 | -999 |
- |
method for measuring thiaminase activity in complex samples. The assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, which is able to use a variety of nucleophiles as cosubstrates. This assay is sensitive and uses readily available chemicals and a visible region spectrophotometer. In addition, the assay can be easily performed in a high-throughput fashion in either 96- or 384-well plates |
684401 |
