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Literature summary for 2.5.1.2 extracted from

  • Hanes, J.W.; Kraft, C.E.; Begley, T.P.
    An assay for thiaminase I in complex biological samples (2007), Anal. Biochem., 368, 33-38.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0211
-
thiamine
-
Paenibacillus thiaminolyticus
0.0211
-
thiamine pH 7.2, temperature not specified in the publication Paenibacillus thiaminolyticus
0.0364
-
4-nitrothiophenolate
-
Paenibacillus thiaminolyticus
0.0364
-
4-nitrothiophenolate pH 7.2, temperature not specified in the publication Paenibacillus thiaminolyticus

Organism

Organism UniProt Comment Textmining
Paenibacillus thiaminolyticus
-
-
-
Paenibacillus thiaminolyticus P45741 recombinant
-

Purification (Commentary)

Purification (Comment) Organism
-
Paenibacillus thiaminolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
a method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. The assay is optimized for high-throughput analysis in a 96-well format with complex biological samples Paenibacillus thiaminolyticus
additional information
-
method for measuring thiaminase activity in complex samples. The assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, which is able to use a variety of nucleophiles as cosubstrates. This assay is sensitive and uses readily available chemicals and a visible region spectrophotometer. In addition, the assay can be easily performed in a high-throughput fashion in either 96- or 384-well plates Paenibacillus thiaminolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine + 4-nitrothiophenolate no activity with thiaminase II Paenibacillus thiaminolyticus 4-methyl-5-(2-hydroxyethyl)thiazole + 2-methyl-5[[(4-nitrophenyl)sulfanyl]methyl]pyrimidin-4-amine
-
?
thiamine + 4-nitrothiophenolate
-
Paenibacillus thiaminolyticus ?
-
?

Synonyms

Synonyms Comment Organism
thiaminase I
-
Paenibacillus thiaminolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
260
-
thiamine
-
Paenibacillus thiaminolyticus
260
-
thiamine pH 7.2, temperature not specified in the publication Paenibacillus thiaminolyticus
297
-
4-nitrothiophenolate
-
Paenibacillus thiaminolyticus
297
-
4-nitrothiophenolate pH 7.2, temperature not specified in the publication Paenibacillus thiaminolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Paenibacillus thiaminolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8380
-
4-nitrothiophenolate pH 7.2, temperature not specified in the publication Paenibacillus thiaminolyticus
12400
-
thiamine pH 7.2, temperature not specified in the publication Paenibacillus thiaminolyticus