KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0211 | - |
thiamine | - |
Paenibacillus thiaminolyticus | |
0.0211 | - |
thiamine | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus | |
0.0364 | - |
4-nitrothiophenolate | - |
Paenibacillus thiaminolyticus | |
0.0364 | - |
4-nitrothiophenolate | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus thiaminolyticus | - |
- |
- |
Paenibacillus thiaminolyticus | P45741 | recombinant | - |
Purification (Comment) | Organism |
---|---|
- |
Paenibacillus thiaminolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
a method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411 nm. This assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. The assay is optimized for high-throughput analysis in a 96-well format with complex biological samples | Paenibacillus thiaminolyticus |
additional information | - |
method for measuring thiaminase activity in complex samples. The assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, which is able to use a variety of nucleophiles as cosubstrates. This assay is sensitive and uses readily available chemicals and a visible region spectrophotometer. In addition, the assay can be easily performed in a high-throughput fashion in either 96- or 384-well plates | Paenibacillus thiaminolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine + 4-nitrothiophenolate | no activity with thiaminase II | Paenibacillus thiaminolyticus | 4-methyl-5-(2-hydroxyethyl)thiazole + 2-methyl-5[[(4-nitrophenyl)sulfanyl]methyl]pyrimidin-4-amine | - |
? | |
thiamine + 4-nitrothiophenolate | - |
Paenibacillus thiaminolyticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thiaminase I | - |
Paenibacillus thiaminolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
260 | - |
thiamine | - |
Paenibacillus thiaminolyticus | |
260 | - |
thiamine | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus | |
297 | - |
4-nitrothiophenolate | - |
Paenibacillus thiaminolyticus | |
297 | - |
4-nitrothiophenolate | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Paenibacillus thiaminolyticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8380 | - |
4-nitrothiophenolate | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus | |
12400 | - |
thiamine | pH 7.2, temperature not specified in the publication | Paenibacillus thiaminolyticus |