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EC Number	Renatured (Commentary)	Reference
3.2.1.140	In vitro refolding, overview. Denatured C-terminally His-tagged enzyme LnbX is refolded in the presence and absence of LnbY and metals Ca2and Mg2+. Purified LnbX is denatured in 6 M guanidine HCl. It is diluted 100fold by adding 50 mM HEPES buffer, pH 7.0, containing various concentrations of LnbY and metal ions 0.0-5.0 mM in a total volume of 0.5 ml, followed by immediate dialysis against HEPES buffer containing or not containing metal ions	732113

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Release 2023.1 (January 2023)