EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    4.1.1.43 | 0.003 |
- |
Pyruvic acid |
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.0058 |
- |
phenylpyruvate |
allosteric enzyme, Hill coefficient 4.7, pH 7.5, temperature not specified in the publication |
746595 |
| 4.1.1.43 | 0.007 |
- |
2-oxobutanoic acid |
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.009 |
- |
2-oxobutanoic acid |
mutant enzyme I335Y, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.027 |
- |
Pyruvic acid |
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.03 |
- |
phenylpyruvate |
mutant enzyme I335Y/M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.035 |
- |
Pyruvic acid |
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.06 |
- |
Pyruvic acid |
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.07 |
- |
Pyruvic acid |
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.315 |
- |
2-oxobutanoic acid |
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
