EC Number   |
|---|
   3.4.21.83 | - |
| 3.4.21.83 | by affinity chromatography and separation of enzyme species A and B |
| 3.4.21.83 | HB101 gene cloned and expressed in JM83 |
| 3.4.21.83 | Ni-NTA agarose column chromatography |
| 3.4.21.83 | purified by using metal-affinity chromatography |
| 3.4.21.83 | purified by using using metal-affinity chromatography |
| 3.4.21.83 | QSepharose column chromatography, ultrafiltration, Ni-NTA-agarose column chromatography, and immobilized basic pancreatic trypsin inhibitor-Sepharose column chromatography |
| 3.4.21.83 | recombinant |
| 3.4.21.83 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) |
| 3.4.21.83 | TALON metal affinity resin column chromatography |
