EC Number |
---|
2.3.1.B43 | - |
2.3.1.B43 | HisTrap column chromatography and Hitrap column chromatography |
2.3.1.B43 | purification of SIRT5 isozymes from HepG2 cells |
2.3.1.B43 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography and gel filtration |
2.3.1.B43 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
2.3.1.B43 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, tag cleavage by TEV protease, reverse cobalt affinity chromatography, and gel filtration |
2.3.1.B43 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain Transetta(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and ultrafiltration |
2.3.1.B43 | recombinant N-terminally StrepII-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration |