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Search Purification (Commentary)

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Results 1 - 9 of 9
EC Number Purification (Commentary) Reference
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54- 14931, 14936, 14937, 487220, 487222, 487226, 487234, 487236, 487239
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54centrifugation, washing in 50 mM potassium phosphate buffer, pH 7.5, resuspension, French press disruption, centrifugation of crude extract and of resulting supernatant 701826
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54gel filtration, Western Blot, heterologously expressed protein 686477
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54native enzyme at pH 6.4, by anion exchange and hydrophobic interaction chromatography, and gel filtration, to homogeneity 675371
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54native enzyme from strain BL21(DE3) by gel filtration and anion exchange chromatography, recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography as single enzyme or as AdhE-Pfl 671620
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54partially 487221
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54quaternary methylamine AP5-ion exchange column chromatography and phenyl Sepharose column chromatography 757586
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54recombinant enzyme 487233, 487237
Show all pathways known for 2.3.1.54Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.54recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography to homogeneity 672694
Results 1 - 9 of 9