Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.54 extracted from

  • Weidner, G.; Sawers, G.
    Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum (1996), J. Bacteriol., 178, 2440-2444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.54

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
pfl gene cloned Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000 85000 SDS-PAGE Streptococcus sanguinis
80000 85000 SDS-PAGE Streptococcus salivarius
85000
-
 2 * 85000, homodimer, SDS-PAGE Escherichia coli
85000
-
 2 * 85000, homodimer, SDS-PAGE Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + CoA Clostridium sp.
-
 acetyl-CoA + formate
-
 ?
pyruvate + CoA Streptococcus sanguinis
-
 acetyl-CoA + formate
-
 ?
pyruvate + CoA Streptococcus salivarius
-
 acetyl-CoA + formate
-
 ?
pyruvate + CoA Clostridium pasteurianum
-
 acetyl-CoA + formate
-
 ?
pyruvate + CoA Clostridium pasteurianum required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 ?
pyruvate + CoA Clostridium butyricum required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 ?
pyruvate + CoA Clostridium kluyveri required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 ?
pyruvate + CoA Escherichia coli catabolic function, leading to production of ATP acetyl-CoA + formate
-
 ?
pyruvate + CoA Enterococcus faecalis catabolic function, leading to production of ATP acetyl-CoA + formate
-
 ?
pyruvate + CoA Streptococcus mutans catabolic function, leading to production of ATP acetyl-CoA + formate
-
 ?
pyruvate + CoA Clostridium pasteurianum DSM 525 required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 ?
pyruvate + CoA Clostridium butyricum DSM 552 required for synthesis of C1 units in anabolism acetyl-CoA + formate
-
 ?
pyruvate + CoA Streptococcus sanguinis DSM 20066
-
 acetyl-CoA + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
 DSM552
-
Clostridium butyricum DSM 552
-
 DSM552
-
Clostridium kluyveri
-
-
-
Clostridium pasteurianum
-
 DSM525
-
Clostridium pasteurianum Q46266 DSM525, nucleotide sequence accession number, GenBank and EMBL
-
Clostridium pasteurianum DSM 525
-
 DSM525
-
Clostridium sp.
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
no activity in Clostridium pasteurianum
-
-
-
Streptococcus mutans
-
-
-
Streptococcus salivarius
-
 ATCC 13419
-
Streptococcus sanguinis
-
 DSM20066
-
Streptococcus sanguinis DSM 20066
-
 DSM20066
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterococcus faecalis
-
 Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Enterococcus faecalis acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Clostridium pasteurianum acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Clostridium butyricum acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Clostridium kluyveri acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Clostridium sp. acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Clostridium sp. acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Streptococcus sanguinis acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Streptococcus sanguinis acetyl-CoA + formate
-
 r
pyruvate + CoA
-
 Streptococcus mutans acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Streptococcus salivarius acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Clostridium pasteurianum acetyl-CoA + formate
-
 r
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium pasteurianum acetyl-CoA + formate
-
 ?
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium butyricum acetyl-CoA + formate
-
 ?
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium kluyveri acetyl-CoA + formate
-
 ?
pyruvate + CoA catabolic function, leading to production of ATP Escherichia coli acetyl-CoA + formate
-
 ?
pyruvate + CoA catabolic function, leading to production of ATP Enterococcus faecalis acetyl-CoA + formate
-
 ?
pyruvate + CoA catabolic function, leading to production of ATP Streptococcus mutans acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Clostridium pasteurianum DSM 525 acetyl-CoA + formate
-
 ?
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium pasteurianum DSM 525 acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Clostridium butyricum DSM 552 acetyl-CoA + formate
-
 r
pyruvate + CoA required for synthesis of C1 units in anabolism Clostridium butyricum DSM 552 acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Streptococcus sanguinis DSM 20066 acetyl-CoA + formate
-
 ?
pyruvate + CoA
-
 Streptococcus sanguinis DSM 20066 acetyl-CoA + formate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 85000, homodimer, SDS-PAGE Escherichia coli
dimer 2 * 85000, homodimer, SDS-PAGE Clostridium pasteurianum