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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 1.14.13.81Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.81cells are centrifuged, sonicated, cell debris removed by centrifugation, membrane fraction separated from the soluble fraction by ultracentrifugation, chlorosome fractioned by sucrose density gradient (15-45%), for SDS-PAGE chlorosome fraction is incubated in methanol, centrifuged, the pellet containing the chlorosome envelop is subjected to SDS-PAGE, protein identification by MALDI-TOF and liquid chromatography-tandem mass spectrometry
Show all pathways known for 1.14.13.81Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.81partial
Show all pathways known for 1.14.13.81Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.81partial, soluble fraction
Show all pathways known for 1.14.13.81Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.81partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants
Show all pathways known for 1.14.13.81Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.81single-subunit O2-dependent cyclase AcsF, recombinant N-terminally His6-tagged enzyme from Escherichia coli strain C43(DE3) by nickel affinity chromatography and gel filtration
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