EC Number   |
Reference |
|---|
    1.13.11.3 | enzymes from Escherichia coli: DEAE-Sepharose Fast Flow column (5.5 x 19 cm), Phenyl-Sepharose CL-4B column (4.5 x 22.5 cm), Sephacryl S-300 column (3 x 97.5 cm) |
671966 |
| 1.13.11.3 | enzymes from Pseudomonas florescens |
671966 |
| 1.13.11.3 | native enzyme 296.8fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography |
725655 |
| 1.13.11.3 | Ni-affinity column chromatography |
741660 |
| 1.13.11.3 | partial |
439481 |
| 1.13.11.3 | protocol for the expression and purification of highly active Pseudomonas putida protocatechuate-3,4-dioxygenase with no detectable nuclease contamination. Purification by nickel affinity chromatography, elution in 2 steps of imidazole concentrations, chromatography fractions are analyzed by SDS-PAGE, fractions of nearly pure enzyme are concentrated and further purified by size exclusion chromatography |
765292 |
| 1.13.11.3 | Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, phenyl-superose HR5/5, Mono Q, type II protocatechuate 3,4-dioxygenase |
439505 |
| 1.13.11.3 | Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, Phenyl-Superose, type I protocatechuate 3,4-dioxygenase |
439505 |
| 1.13.11.3 | Q-Sepharose FF, ammonium sulfate, Superdex 200, Fractogel, type II protocatechuate 3,4-dioxygenase |
439505 |
| 1.13.11.3 | recombinant enzyme |
439512 |
