1.13.11.3	-	439480, 439482, 439483, 439498, 439502, 439503, 439506, 439507, 665455, 666009, 685485, 763959	
1.13.11.3	a highly active enzyme without nuclease contaminations is purified by Ni-NTA affinity chromatography and size exclusion chromatography for prolonged single-molecule fluorescence imaging	763879	
1.13.11.3	ammonium sulfate, DEAE-cellulose	439488	
1.13.11.3	ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose	439501	
1.13.11.3	ammonium sulfate, DEAE-cellulose, Sephadex G-200	439500	
1.13.11.3	ammonium sulfate, DEAE-Sepharose, octyl-Sepharose, Phenyl-sepharose, recombinant enzyme	439508	
1.13.11.3	ammonium sulfate, DEAE-Sepharose, Phenyl-Sepharose	439484	
1.13.11.3	ammonium sulfate, Q-Sepharose, partial purification	439514	
1.13.11.3	ammonium sulfate, Sephacryl S 300, DEAE-Sephadex A50	439499	
1.13.11.3	cells harvested by centrifugation, washed twice with 50 mM sodium phosphate buffer, pH 7.0 containing 10% glycerol, disruption with solicitor, centrifuged, supernatant stored at -20°C	697244	
1.13.11.3	enzymes from Escherichia coli: DEAE-Sepharose Fast Flow column (5.5 x 19 cm), Phenyl-Sepharose CL-4B column (4.5 x 22.5 cm), Sephacryl S-300 column (3 x 97.5 cm)	671966	
1.13.11.3	enzymes from Pseudomonas florescens	671966	
1.13.11.3	native enzyme 296.8fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography	725655	
1.13.11.3	Ni-affinity column chromatography	741660	
1.13.11.3	partial	439481	
1.13.11.3	protocol for the expression and purification of highly active Pseudomonas putida protocatechuate-3,4-dioxygenase with no detectable nuclease contamination. Purification by nickel affinity chromatography, elution in 2 steps of imidazole concentrations, chromatography fractions are analyzed by SDS-PAGE, fractions of nearly pure enzyme are concentrated and further purified by size exclusion chromatography	765292	
1.13.11.3	Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, phenyl-superose HR5/5, Mono Q, type II protocatechuate 3,4-dioxygenase	439505	
1.13.11.3	Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, Phenyl-Superose, type I protocatechuate 3,4-dioxygenase	439505	
1.13.11.3	Q-Sepharose FF, ammonium sulfate, Superdex 200, Fractogel, type II protocatechuate 3,4-dioxygenase	439505	
1.13.11.3	recombinant enzyme	439512