EC Number   |
Application |
Reference |
|---|
   3.1.11.2 | analysis |
a combined treatment of nucleosomes with micrococcal nuclease and exonuclease III overcomes micrococcal nuclease sequence preference and produces nucleosomal DNA trimmed symmetrically and precisely at the core/linker junctions regardless of the underlying DNA sequence. Combined micrococcal nuclease/exonuclease III digestion can be applied to in situ chromatin for unbiased genome-wide mapping of nucleosome positions that is not ifluenced by DNA sequences at the core/linker junctions. The same approach can be also used for the precise mapping of the extent of linker DNA protection by H1 and other protein factors associated with nucleosome linkers |
730213 |
| 3.1.11.2 | analysis |
analysis of 3'-terminal nucleotide sequences of duplex DNA. Under selected conditions the enzyme can be used to remove a small defined number of nucleotides from each 3'-terminus of duplex DNA |
133966 |
| 3.1.11.2 | analysis |
cell cycle profiling by image and flow cytometry, optimised protocol for the detection of replicational activity using 5-bromo-2'-deoxyuridine, low concentration of hydrochloric acid and exonuclease III, method evaluation and optimization, detailed overview |
751965 |
| 3.1.11.2 | analysis |
development of a graphene oxide-based fluorescent aptasensor for adenosine detection by employing exonuclease III as a signal amplifying element. In the presence of adenosine, the adenosine aptamers associate with the targets, which leads to the formation of duplex DNAs between the cDNAs and the signal probes. Exonuclease III thereafter can digest the duplex DNAs from 3' blunt terminus of signal probes, liberating the fluorophore. The biosensor exhibits an ultrahigh sensitivity and holds a versatile platform for clinical diagnostics, molecular biology and drug developments |
729447 |
| 3.1.11.2 | analysis |
enzymatically amplified surface plasmon resonance imaging detection of DNA by exonuclease III digestion of DNA microarrays. Through the use of ExoIII in conjunction with DNA microarrays, a 100-1000 improvement in the detection limit for the multiplexed surface plasmon resonance imaging detection of 16-mer oligonucleotides is achieved. This enhancement is not as great as RNase H amplification with RNA microarrays. The major advantage of ExoIII amplification compared to RNase H is that the additional difficulty in preparing and handling RNA microarrays is avoided. The ExoIII enzymatic amplification process can be used with the more robust and cost-effective DNA microarrays that are currently applied in research areas such as gene analysis and medical diagnostics. Most DNA sequences can be detected since the activity of ExoIII is not sequence dependent |
663627 |
| 3.1.11.2 | synthesis |
enzyme is used for purification of eucaryotic extrachromosomal circular DNAs using exonuclease III |
133975 |
| 3.1.11.2 | analysis |
identification of DNA bonding sites of two pyrrolobenzodiazepine derivatives - tomamycin and anthramycin |
133972 |
| 3.1.11.2 | molecular biology |
in contrast to DNA cloning utilizing in vitro recombination, some strains of Escherichia coli can take up linear double-stranded vectors, insert DNA fragments, and assemble them in vivo involving exonulcease III. The ends of these linear DNA fragments must contain 20 to 50 bp of overlapping homologous sequences. Improved protocols for in vivo cloning have realized a high level of usability comparable to that by in vitro recombination reactions, but using the exonuclease III, it is only necessary to introduce PCR products into Escherichia coli for the in vivo cloning |
750978 |
| 3.1.11.2 | analysis |
restriction analysis of the prototype strain of enteric adenovirus type 41 using exonulease |
133971 |
| 3.1.11.2 | medicine |
study of a novel strategy using enzyme for direct sequencing of double-stranded DNA by MALDI-TOF mass spectroscopy |
653385 |
