Application | Comment | Organism |
---|---|---|
medicine | study of a novel strategy using enzyme for direct sequencing of double-stranded DNA by MALDI-TOF mass spectroscopy | Escherichia coli |
medicine | the enzyme is helpful for PCR amplification of long DNA templates | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates | four activities of enzyme: 3-5 exonuclease, RNase H, 3-phosphatase and AP endonuclease | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | the enzyme degrades the phosphodiester bond from the 3 end of double-stranded DNA | Escherichia coli | deoxynucleoside 5'-phosphate | - |
? |