EC Number   |
Metals/Ions |
Reference  |
|---|
   3.6.5.6 | Mg2+ |
required for activity, strong increase which reaches a plateau at 4 mM MgCl2 |
644203 |
| 3.6.5.6 | Na+ |
required for nocodazole induced GTPase activity |
644206 |
| 3.6.5.6 | Ca2+ |
induction of the GTPase activity of brain tubulin, inhibition of tubulin assembly, prevents tubulin polymerization and induces rapid depolymerization of preformed microtubules |
644207 |
| 3.6.5.6 | Gd3+ |
stimulates the GTPase activity of brain tubulin, inhibits tubulin polymerization and induce microtubule depolymerization |
644207 |
| 3.6.5.6 | Nd3+ |
stimulates the GTP hydrolysis, inhibits tubulin polymerization and induces microtubule depolymerization |
644207 |
| 3.6.5.6 | Mg2+ |
- |
687396, 699587 |
| 3.6.5.6 | K+ |
K+ ion at the GTP binding site allows the positioning of one water molecule that interacts with catalytic residues D235 and D238, K+ ion stabilizes dimer (molecular dynamics simulations) |
699587 |
| 3.6.5.6 | Na+ |
Na+ destabilizes the dimer and does not allow the positioning of the catalytic water molecule (molecular dynamics simulations) |
699587 |
| 3.6.5.6 | Mg2+ |
required |
733253, 733717, 756202, 756504 |
| 3.6.5.6 | Co2+ |
stimulates, can partially substitute for Mg2+ |
733983 |
