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Literature summary for 3.6.5.6 extracted from
- Studies on the nocodazole-induced GTPase activity of tubulin (1996), Arch. Biochem. Biophys., 336, 130-138.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Colchicine | induces GTPase activity | Bos taurus |  |
| nocodazole | induced by this antimitotic drug, GTP hydrolysis rate stimulated four-to fivefold | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | inhibits GTP hydrolysis, but not the assembly reaction | Bos taurus | |
| N-ethylmaleimide | inhibits GTP hydrolysis, but not the assembly reaction | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | required for nocodazole induced GTPase activity | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Bos taurus | - |
GDP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, frozen as pellets in liquid nitrogen | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | hydrolyzed at a rate about half as fast like GTP | Bos taurus | ADP + phosphate | - |
? | |
| GTP + H2O | - |
Bos taurus | GDP + phosphate | - |
? | |
| GTP + H2O | inverse isotope effect, reaction rate in D2O is twice the rate in H2O | Bos taurus | GDP + phosphate | - |
? | |
| ITP + H2O | hydrolyzed at equivalent rates like GTP | Bos taurus | IDP + phosphate | - |
? | |
| XTP + H2O | hydrolyzed at a rate about half as fast like GTP | Bos taurus | XDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 37 | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.2 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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