EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.4.1.10 | 47.81 |
- |
D-glucose |
pH 6.0, 30°C, recombinant enzyme |
704960 |
2.4.1.10 | -999 |
- |
more |
- |
671507 |
2.4.1.10 | -999 |
- |
more |
2.3 mM (sucrose) total, mutant enzyme S173A |
678072 |
2.4.1.10 | -999 |
- |
more |
29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site |
678072 |
2.4.1.10 | -999 |
- |
more |
31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type |
678072 |
2.4.1.10 | -999 |
- |
more |
32 mM (sucrose), 25°C, pH 5.0, total activity |
687775 |
2.4.1.10 | -999 |
- |
more |
35.1 mM (sucrose) total, mutant enzyme N252H |
678072 |
2.4.1.10 | -999 |
- |
more |
4.1 mM (sucrose) total, mutant enzyme N252A |
678072 |
2.4.1.10 | -999 |
- |
more |
480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type |
678072 |
2.4.1.10 | -999 |
- |
more |
51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity |
678072 |