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EC Number
Inhibitors
Commentary
Structure
3.1.21.4
ammonium sulfate
-
3.1.21.4
Co2+
complete inhibition at 1 mM
3.1.21.4
cucurbit[6]uril
cucurbiturils are supramolecular inhibitors of DNA restriction by type II endonucleases. They inhibit the enzymatically catalyzed restriction of plasmids and linear DNA
3.1.21.4
cucurbit[7]uril
cucurbiturils are supramolecular inhibitors of DNA restriction by type II endonucleases. They inhibit the enzymatically catalyzed restriction of plasmids and linear DNA
3.1.21.4
EDTA
-
3.1.21.4
high ionic strength
irreversible inactivation
3.1.21.4
K+
above 100 mM, significant inhibition
3.1.21.4
KCl
above 25 mM
3.1.21.4
Mn2+
complete inhibition at 3 mM
3.1.21.4
more
increasing proportions of uracil in DNA substrates increases inhibition of restriction enzyme digests
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