Literature summary for 7.4.2.8 extracted from

Burgess, J.L.; Jones, H.B.; Kumar, P.; Toth, R.T.; Middaugh, C.R.; Antony, E.; Dickenson, N.E.
Spa47 is an oligomerization-activated type three secretion system (T3SS) ATPase from Shigella flexneri (2016), Protein Sci., 25, 1037-1048 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Tuner (DE3) cells	Shigella flexneri

Protein Variants

Protein Variants	Comment	Organism
K165A	inactive	Shigella flexneri

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Shigella flexneri	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10 mM used in assay conditions	Shigella flexneri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + cellular protein[side 1]	Shigella flexneri	-	ADP + phosphate + cellular protein[side 2]	-	?
ATP + H2O + cellular protein[side 1]	Shigella flexneri 2457T	-	ADP + phosphate + cellular protein[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Shigella flexneri	-	-	-
Shigella flexneri 2457T	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
chitin column chromatography, Q-Sepharose column chromatography, and Superdex 16/600 gel filtration	Shigella flexneri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + cellular protein[side 1]	-	Shigella flexneri	ADP + phosphate + cellular protein[side 2]	-	?
ATP + H2O + cellular protein[side 1]	-	Shigella flexneri 2457T	ADP + phosphate + cellular protein[side 2]	-	?

Subunits

Subunits	Comment	Organism
?	x * 50000, SDS-PAGE	Shigella flexneri

Synonyms

Synonyms	Comment	Organism
Spa47	-	Shigella flexneri
T3SS ATPase	-	Shigella flexneri
type three secretion system ATPase	-	Shigella flexneri

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.18	-	ATP	at pH 7.9 and 25°C	Shigella flexneri

General Information

General Information	Comment	Organism
physiological function	the enzyme is necessary for cellular invasion . The enzyme provides the energy for the type three secretion apparatus which penetrates the host cell membrane and provides a unidirectional conduit for injection of effectors into host cells	Shigella flexneri

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Release 2023.1 (January 2023)