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Literature summary for 7.4.2.8 extracted from
- MxiN differentially regulates monomeric and oligomeric species of the Shigella type three secretion system ATPase Spa47 (2018), Biochemistry, 57, 2266-2277 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Tuner (DE3) cells | Shigella flexneri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.114 | - |
ATP | at pH 7.9 and 25°C | Shigella flexneri |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM MgCl2 used in assay conditions | Shigella flexneri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + cellular protein[side 1] | Shigella flexneri | - |
ADP + phosphate + cellular protein[side 2] | - |
? | |
| ATP + H2O + cellular protein[side 1] | Shigella flexneri 2457T | - |
ADP + phosphate + cellular protein[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shigella flexneri | - |
- |
- |
| Shigella flexneri 2457T | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chitin resin column chromatography, Q Sepharose column chromatography, and Superdex 200 gel filtration | Shigella flexneri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + cellular protein[side 1] | - |
Shigella flexneri | ADP + phosphate + cellular protein[side 2] | - |
? | |
| ATP + H2O + cellular protein[side 1] | - |
Shigella flexneri 2457T | ADP + phosphate + cellular protein[side 2] | - |
? | |
| additional information | MxiN interacts with and differentially regulates the activity of the enzyme in vivo | Shigella flexneri | ? | - |
? | |
| additional information | MxiN interacts with and differentially regulates the activity of the enzyme in vivo | Shigella flexneri 2457T | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 48000, SDS-PAGE | Shigella flexneri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Spa47 | - |
Shigella flexneri |
| T3SS ATPase | - |
Shigella flexneri |
| type three secretion system ATPase | - |
Shigella flexneri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.84 | - |
ATP | at pH 7.9 and 25°C | Shigella flexneri | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.5 | - |
ATP | at pH 7.9 and 25°C | Shigella flexneri | |
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Release 2023.1 (January 2023)
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