Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.4.2.8 extracted from

  • Bzdzion, L.; Krezel, H.; Wrzeszcz, K.; Grzegorek, I.; Nowinska, K.; Chodaczek, G.; Swietnicki, W.
    Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli (2017), Acta Biochim. Pol., 64, 49-63 .
    View publication on PubMed
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(2S)-1-(1H-indol-3-yl)-1-oxopropan-2-yl 2-(4-acetamidophenyl)-1,3-dioxo-2,3-dihydro-1H-isoindole-5-carboxylate 22% inhibition at 0.1mM Escherichia coli
2-(2-amino-2-oxoethoxy)-N-(3-oxo-3,4-dihydro-2H-1,4-benzoxazin-6-yl)benzamide 2.2% inhibition at 0.1mM Escherichia coli
2-(4-ethylphenyl)-5-(hydroxymethyl)-N-(4-phenylbutyl)-2H-1,2,3-triazole-4-carboxamide 22% inhibition at 0.1mM Escherichia coli
2-(4-[2-[(2-amino-2-oxoethyl)sulfanyl]benzoyl]piperazin-1-yl)-N-(4-fluorophenyl)acetamide
-
 Escherichia coli
2-oxo-2-(3-oxo-3,4-dihydroquinoxalin-1(2H)-yl)ethyl 4-[(1,3-benzothiazol-2-yl)sulfanyl]butanoate 26% inhibition at 0.1mM; 29% inhibition at 0.1mM Escherichia coli
2-oxo-2-[4-(trifluoromethoxy)anilino]ethyl 2-hydroxy-5-methylbenzoate 32% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-(1H-indol-4-yl)benzamide 3% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-(2,3-dioxo-1,2,3,4-tetrahydroquinoxalin-6-yl)benzamide competitive inhibitor, % inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-(2-methyl-1-phenyl-1H-benzimidazol-5-yl)benzamide competitive inhibition, 17% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-6-yl)benzamide competitive inhibition, 30% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-phenylbenzamide
-
 Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[1-(cyanomethyl)-2,3-dihydro-1H-indol-7-yl]benzamide competitive inhibitor, % inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[2-(propan-2-yl)-1,3-benzoxazol-5-yl]benzamide
-
 Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[3-(6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepin-3-yl)phenyl]benzamide 1% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[3-(trifluoromethyl)phenyl]benzamide
-
 Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[3-[(methanesulfonyl)amino]-4-methylphenyl]benzamide
-
 Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-(1H-imidazol-1-yl)phenyl]benzamide
-
 Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-(2-oxopyrrolidin-1-yl)phenyl]benzamide
-
 Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-(piperidin-1-yl)phenyl]benzamide competitive inhibition, 12% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-(trifluoromethyl)phenyl]benzamide competitive inhibition, 16% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-methoxy-3-(piperidine-1-sulfonyl)phenyl]benzamide 2% inhibition at 0.1mM Escherichia coli
2-[(2-amino-2-oxoethyl)sulfanyl]-N-[[4-(piperidine-1-carbonyl)phenyl]methyl]benzamide
-
 Escherichia coli
2-[[2-(4-benzoylpiperazine-1-carbonyl)phenyl]sulfanyl]acetamide
-
 Escherichia coli
2-[[2-(cyclopropylamino)-2-oxoethyl]sulfanyl]-N-(3-oxo-3,4-dihydro-2H-1,4-benzoxazin-6-yl)benzamide 1% inhibition at 0.1mM Escherichia coli
3-(4-fluorophenyl)-7-(3-hydroxy-4-methoxyphenyl)-3,4,6,7-tetrahydro-5H-imidazo[4,5-b]pyridin-5-one 14% inhibition at 0.1mM Escherichia coli
3-[(3R)-2-hydroxy-5-oxo-4,5-dihydro-3H-1,4-benzodiazepin-3-yl]-N-[(2S)-6-methylheptan-2-yl]propanamide 26% inhibition at 0.1mM Escherichia coli
ethyl 5-methyl-1-[3-[4-(2-oxo-1,3-benzoxazol-3(2H)-yl)butanamido]phenyl]-1H-pyrazole-4-carboxylate 14% inhibition at 0.1mM Escherichia coli
ethyl [2-(2-[[2-(propan-2-yl)quinazolin-4-yl]sulfanyl]acetamido)-1,3-thiazol-4-yl]acetate competitive inhibitor, % inhibition at 0.1mM Escherichia coli
methyl 4-[2-[(2-amino-2-oxoethyl)sulfanyl]benzamido]-1H-indazole-3-carboxylate competitive inhibitor, % inhibition at 0.1mM Escherichia coli
N-(2-[[(2R)-2-(carbamoylamino)pentanoyl]amino]ethyl)benzamide 2% inhibition at 0.1mM Escherichia coli
N-[2-[(2-amino-2-oxoethyl)sulfanyl]phenyl]-1-benzofuran-2-carboxamide 4% inhibition at 0.1mM Escherichia coli
N-[2-[(2-amino-2-oxoethyl)sulfanyl]phenyl]-1-benzothiophene-2-carboxamide 5% inhibition at 0.1mM Escherichia coli
N-[2-[(2-amino-2-oxoethyl)sulfanyl]phenyl]naphthalene-2-carboxamide noncompetitive inhibition, 15% inhibition at 0.1mM Escherichia coli
N-[4-(2-oxo-1,2,3,4-tetrahydroquinolin-6-yl)-1,3-thiazol-2-yl]-3-[(1,3-thiazol-4-yl)methoxy]benzamide 16% inhibition at 0.1mM Escherichia coli
N-[4-(2-[[5-(furan-2-yl)-4-oxothieno[2,3-d]pyrimidin-3(4H)-yl]amino]-2-oxoethyl)-1,3-thiazol-2-yl]benzamide 16% inhibition at 0.1mM Escherichia coli
WEN01-01 competitive inhibitor Escherichia coli
WEN01-04 competitive inhibitor Escherichia coli
WEN04-34 competitive inhibitor Escherichia coli
WEN05-03 competitive inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 ATP at pH 8.5 and 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM MgCl2 used in assay conditions Escherichia coli
Na+ 150 mM NaCl used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + cellular protein[side 1] Escherichia coli
-
 ADP + phosphate + cellular protein[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli B7UMA6 EPEC
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Ni-agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + cellular protein[side 1]
-
 Escherichia coli ADP + phosphate + cellular protein[side 2]
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, GST-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
EscN
-
 Escherichia coli
type III secretion system ATPase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.289
-
 ATP at pH 8.5 and 25°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.016
-
 2-[(2-amino-2-oxoethyl)sulfanyl]-N-(2,3-dioxo-1,2,3,4-tetrahydroquinoxalin-6-yl)benzamide at pH 8.5 and 25°C Escherichia coli
0.016
-
 WEN05-03 at pH 8.5 and 25°C Escherichia coli
0.046
-
 ethyl [2-(2-[[2-(propan-2-yl)quinazolin-4-yl]sulfanyl]acetamido)-1,3-thiazol-4-yl]acetate at pH 8.5 and 25°C Escherichia coli
0.046
-
 WEN04-34 at pH 8.5 and 25°C Escherichia coli
0.255
-
 2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-(piperidin-1-yl)phenyl]benzamide at pH 8.5 and 25°C Escherichia coli
0.44
-
 2-[(2-amino-2-oxoethyl)sulfanyl]-N-[1-(cyanomethyl)-2,3-dihydro-1H-indol-7-yl]benzamide at pH 8.5 and 25°C Escherichia coli
0.44
-
 WEN01-04 at pH 8.5 and 25°C Escherichia coli
0.47
-
 methyl 4-[2-[(2-amino-2-oxoethyl)sulfanyl]benzamido]-1H-indazole-3-carboxylate at pH 8.5 and 25°C Escherichia coli
0.47
-
 WEN01-01 at pH 8.5 and 25°C Escherichia coli
0.475
-
 2-[(2-amino-2-oxoethyl)sulfanyl]-N-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-6-yl)benzamide at pH 8.5 and 25°C Escherichia coli
0.5
-
 2-[(2-amino-2-oxoethyl)sulfanyl]-N-[4-(trifluoromethyl)phenyl]benzamide at pH 8.5 and 25°C Escherichia coli
0.55
-
 2-[(2-amino-2-oxoethyl)sulfanyl]-N-(2-methyl-1-phenyl-1H-benzimidazol-5-yl)benzamide at pH 8.5 and 25°C Escherichia coli
3.5
-
 N-[2-[(2-amino-2-oxoethyl)sulfanyl]phenyl]naphthalene-2-carboxamide at pH 8.5 and 25°C Escherichia coli