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Literature summary for 7.4.2.8 extracted from

  • Stone, C.B.; Johnson, D.L.; Bulir, D.C.; Gilchrist, J.D.; Mahony, J.B.
    Characterization of the putative type III secretion ATPase CdsN (Cpn0707) of Chlamydophila pneumoniae (2008), J. Bacteriol., 190, 6580-6588.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expresssion of GST-tagged full-length CdsN and a C-terminal truncation mutant of CdsN in Escherichia coli strain BL21(DE3) Chlamydia pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information the GST-tagged C-terminal truncation mutant of CdsN possesses ATPase activity Chlamydia pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme shows positive cooperativity Chlamydia pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Chlamydia pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48100
-
 x * 48100, about, sequence calculation, SDS-PAGE, CdsN forms oligomers and high-molecular-weight multimers, possibly dodecamers Chlamydia pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Chlamydia pneumoniae the type III secretion system is dependent on ATPase activity, which catalyzes the unfolding of proteins and the secretion of effector proteins through the injectisome. CdsN, Cpn0707, is the T3S ATPase. CdsN interacts with CdsD, CdsL, CdsQ, and CopN, four putative structural components of the T3S system, CdsN also interacts with an unannotated protein, Cpn0706, a putative CdsN chaperone, overview ADP + phosphate
-
 ?
ATP + H2O Chlamydia pneumoniae CWL-029 / ATCC VR1310 the type III secretion system is dependent on ATPase activity, which catalyzes the unfolding of proteins and the secretion of effector proteins through the injectisome. CdsN, Cpn0707, is the T3S ATPase. CdsN interacts with CdsD, CdsL, CdsQ, and CopN, four putative structural components of the T3S system, CdsN also interacts with an unannotated protein, Cpn0706, a putative CdsN chaperone, overview ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydia pneumoniae
-
-
-
Chlamydia pneumoniae CWL-029 / ATCC VR1310
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged full-length CdsN and CdsN C-terminal truncation mutant from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Chlamydia pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
 wild-type full-length CdsN and C-terminal truncation mutant of CdsN, ATPase activity Chlamydia pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Chlamydia pneumoniae ADP + phosphate
-
 ?
ATP + H2O the type III secretion system is dependent on ATPase activity, which catalyzes the unfolding of proteins and the secretion of effector proteins through the injectisome. CdsN, Cpn0707, is the T3S ATPase. CdsN interacts with CdsD, CdsL, CdsQ, and CopN, four putative structural components of the T3S system, CdsN also interacts with an unannotated protein, Cpn0706, a putative CdsN chaperone, overview Chlamydia pneumoniae ADP + phosphate
-
 ?
ATP + H2O
-
 Chlamydia pneumoniae CWL-029 / ATCC VR1310 ADP + phosphate
-
 ?
ATP + H2O the type III secretion system is dependent on ATPase activity, which catalyzes the unfolding of proteins and the secretion of effector proteins through the injectisome. CdsN, Cpn0707, is the T3S ATPase. CdsN interacts with CdsD, CdsL, CdsQ, and CopN, four putative structural components of the T3S system, CdsN also interacts with an unannotated protein, Cpn0706, a putative CdsN chaperone, overview Chlamydia pneumoniae CWL-029 / ATCC VR1310 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 48100, about, sequence calculation, SDS-PAGE, CdsN forms oligomers and high-molecular-weight multimers, possibly dodecamers Chlamydia pneumoniae

Synonyms

Synonyms Comment Organism
CdsN
-
 Chlamydia pneumoniae
Cpn0707
-
 Chlamydia pneumoniae
type III secretion ATPase
-
 Chlamydia pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Chlamydia pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Chlamydia pneumoniae

pI Value

Organism Comment pI Value Maximum pI Value
Chlamydia pneumoniae recombinant GST-tagged enzyme, sequence calculation
-
 6.1