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Literature summary for 7.4.2.8 extracted from

  • Jakovljevic, V.; Leonardy, S.; Hoppert, M.; Sogaard-Andersen, L.
    PilB and PilT are ATPases acting antagonistically in type IV pilus function in Myxococcus xanthus (2008), J. Bacteriol., 190, 2411-2421.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain JM109, expression of GFP-tagged wild-type PilB and PilT in strain DK1622 Myxococcus xanthus

Protein Variants

Protein Variants Comment Organism
E205A a Walker B box substitution in PilT, leads to strong reduction of ATPase activity due to direct interference with ATP hydrolysis Myxococcus xanthus
E391A a Walker B box substitution in PilB, leads to strong reduction of ATPase activity due to direct interference with ATP hydrolysis Myxococcus xanthus
K137A a Walker A box substitution in PilT, leads to abolishment of ATPase activity due to indirect interference withe ATP binding Myxococcus xanthus
K327A a Walker A box substitution in PilB, leads to strong reduction of ATPase activity due to indirect interference withe ATP binding Myxococcus xanthus
additional information construction of diverse Walker A and Walker B boxe mutants, PilB as well as PilT ATPase activity is abolished in vitro by replacement of conserved residues in the Walker A and Walker B boxes that are involved in ATP binding and hydrolysis, respectively, cell phenotypes, overview Myxococcus xanthus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm PilB and PilT Myxococcus xanthus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 1 * 62000, recombinant His-tagged PilB, SDS-PAGE, x * 63800, about, His6-tagged PilB, sequence calculation, 1 * 39000, recombinant His-tagged PilT, SDS-PAGE, 1 * 41000, about, His6-tagged PilT, sequence calculation Myxococcus xanthus
40000
-
 PilT, gel filtration Myxococcus xanthus
41000
-
 1 * 62000, recombinant His-tagged PilB, SDS-PAGE, x * 63800, about, His6-tagged PilB, sequence calculation, 1 * 39000, recombinant His-tagged PilT, SDS-PAGE, 1 * 41000, about, His6-tagged PilT, sequence calculation Myxococcus xanthus
60000
-
 PilB, gel filtration Myxococcus xanthus
62000
-
 1 * 62000, recombinant His-tagged PilB, SDS-PAGE, x * 63800, about, His6-tagged PilB, sequence calculation, 1 * 39000, recombinant His-tagged PilT, SDS-PAGE, 1 * 41000, about, His6-tagged PilT, sequence calculation Myxococcus xanthus
63800
-
 1 * 62000, recombinant His-tagged PilB, SDS-PAGE, x * 63800, about, His6-tagged PilB, sequence calculation, 1 * 39000, recombinant His-tagged PilT, SDS-PAGE, 1 * 41000, about, His6-tagged PilT, sequence calculation Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Myxococcus xanthus PilB and PilT of the type IV pili, T4P, system in Myxococcus xanthus have ATPase activity acting at distinct steps in the T4P extension/retraction cycle in vivo ADP + phosphate
-
 ?
ATP + H2O Myxococcus xanthus DK 1622 PilB and PilT of the type IV pili, T4P, system in Myxococcus xanthus have ATPase activity acting at distinct steps in the T4P extension/retraction cycle in vivo ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-
Myxococcus xanthus DK 1622
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially by subcellular fractionation of wild-type strain DK1622 expressing GFP-tagged wild-type PilB and PilT, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Myxococcus xanthus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0029
-
 recombinant His6-tagged PilB Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O PilB and PilT of the type IV pili, T4P, system in Myxococcus xanthus have ATPase activity acting at distinct steps in the T4P extension/retraction cycle in vivo Myxococcus xanthus ADP + phosphate
-
 ?
ATP + H2O PilB and PilT of the type IV pili, T4P, system in Myxococcus xanthus have ATPase activity in vitro Myxococcus xanthus ADP + phosphate
-
 ?
ATP + H2O PilB and PilT of the type IV pili, T4P, system in Myxococcus xanthus have ATPase activity acting at distinct steps in the T4P extension/retraction cycle in vivo Myxococcus xanthus DK 1622 ADP + phosphate
-
 ?
ATP + H2O PilB and PilT of the type IV pili, T4P, system in Myxococcus xanthus have ATPase activity in vitro Myxococcus xanthus DK 1622 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000, recombinant His-tagged PilB, SDS-PAGE, x * 63800, about, His6-tagged PilB, sequence calculation, 1 * 39000, recombinant His-tagged PilT, SDS-PAGE, 1 * 41000, about, His6-tagged PilT, sequence calculation Myxococcus xanthus
More PilB and PilT proteins do not form oligomers under all conditions tested, overview Myxococcus xanthus

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the secretion ATPase superfamily of proteins Myxococcus xanthus
PilB
-
 Myxococcus xanthus
PilT
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Myxococcus xanthus