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Literature summary for 7.4.2.8 extracted from
- Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU (2008), Microbiology, 154, 114-126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes pilB, pilT and pilU from strain PAK, expression of wild-type and mutant enzymes in Escherichia coli strain Bl21(DE3). Expression of YFP-PilT, YFP-PilB, and YFP-PilU fusion enzymes, which retain their characteristic pattern of polar localization, with the exception of the Walker A mutant of YFP-PilT | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D160N | mutation in the Walker A motif of PilT, and in PilU | Pseudomonas aeruginosa |
| D355N | mutation of PilB | Pseudomonas aeruginosa |
| E159Q | mutation in the Walker A motif of PilT, and in PilU | Pseudomonas aeruginosa |
| E163Q | mutation in the Walker A motif of PilT, and in PilU | Pseudomonas aeruginosa |
| E204 | mutation in the Walker B motif of PilT, and in PilU, inactive PilT mutant | Pseudomonas aeruginosa |
| E354Q | mutation of PilB | Pseudomonas aeruginosa |
| E358Q | mutation of PilB | Pseudomonas aeruginosa |
| E396Q | mutation of PilB | Pseudomonas aeruginosa |
| G135S | mutation in the Asp Box of PilT, and in PilU, inactive PilT mutant | Pseudomonas aeruginosa |
| G331S | mutation of PilB | Pseudomonas aeruginosa |
| H222A | mutation in the His Box of PilT, and in PilU | Pseudomonas aeruginosa |
| H229A | mutation in the His Box of PilT, and in PilU | Pseudomonas aeruginosa |
| H414A | mutation of PilB | Pseudomonas aeruginosa |
| H421A | mutation of PilB | Pseudomonas aeruginosa |
| additional information | a constructed YFP-PilB construct does not complement a pilB mutant, mutation of conserved Walker A or Walker B residues in any of PilB, PilT and PilU ATPases abrogates twitching motility, and for the Walker A mutant of PilT causes loss of polar localization, overview | Pseudomonas aeruginosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pseudomonas aeruginosa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Pseudomonas aeruginosa | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Pseudomonas aeruginosa PAK | - |
ADP + phosphate | - |
? | |
| additional information | Pseudomonas aeruginosa | PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily, and power pilus extension and retraction, respectively, while the unipolar PilT paralogue PilU supports pilus retraction in an unknown manner. In all three proteins, the third acidic residue in the Asp Box and the second His of the His Box are crucial for function, mutation of these residues causes loss of PilT ATPase activity in vitro | ? | - |
? | |
| additional information | Pseudomonas aeruginosa PAK | PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily, and power pilus extension and retraction, respectively, while the unipolar PilT paralogue PilU supports pilus retraction in an unknown manner. In all three proteins, the third acidic residue in the Asp Box and the second His of the His Box are crucial for function, mutation of these residues causes loss of PilT ATPase activity in vitro | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
genes pilB, pilT and pilU | - |
| Pseudomonas aeruginosa PAK | - |
genes pilB, pilT and pilU | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00108 | - |
recombinant PilT mutant H229A | Pseudomonas aeruginosa |
| 0.00275 | - |
recombinant PilT mutant E163Q | Pseudomonas aeruginosa |
| 0.005 | - |
recombinant wild-type PilU | Pseudomonas aeruginosa |
| 0.0074 | - |
recombinant wild-type PilT | Pseudomonas aeruginosa |
| 0.0108 | - |
recombinant wild-type PilB | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Pseudomonas aeruginosa | ADP + phosphate | - |
? | |
| ATP + H2O | the enzyme shows requirement for three invariant acidic residues in the Walker Asp Box motif, and for two invariant His residues in the His Box motif, structure-function analysis and modelling, overview. The Walker A motif is involved in ATP binding, while the Walker B motif is involved in the hydrolysis of ATP | Pseudomonas aeruginosa | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Pseudomonas aeruginosa PAK | ADP + phosphate | - |
? | |
| ATP + H2O | the enzyme shows requirement for three invariant acidic residues in the Walker Asp Box motif, and for two invariant His residues in the His Box motif, structure-function analysis and modelling, overview. The Walker A motif is involved in ATP binding, while the Walker B motif is involved in the hydrolysis of ATP | Pseudomonas aeruginosa PAK | ADP + phosphate | - |
? | |
| additional information | PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily, and power pilus extension and retraction, respectively, while the unipolar PilT paralogue PilU supports pilus retraction in an unknown manner. In all three proteins, the third acidic residue in the Asp Box and the second His of the His Box are crucial for function, mutation of these residues causes loss of PilT ATPase activity in vitro | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily, and power pilus extension and retraction, respectively, while the unipolar PilT paralogue PilU supports pilus retraction in an unknown manner. In all three proteins, the third acidic residue in the Asp Box and the second His of the His Box are crucial for function, mutation of these residues causes loss of PilT ATPase activity in vitro | Pseudomonas aeruginosa PAK | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | secretion NTPases of the type II secretion and type IV pili systems, including PilB, PilT and PilU, function as toroidal homohexamers and, in addition toWalker A and Walker B motifs, contain unique Asp Box and His Box motifs | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily | Pseudomonas aeruginosa |
| PilB | - |
Pseudomonas aeruginosa |
| PilT | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas aeruginosa |
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